Human Recombinant IL10RA protein, His Tag (V2LY-0526-LY4714)

Name: Human Recombinant IL10RA protein, His Tag
SKU: V2LY-0526-LY4714
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant IL10RA protein, His Tag consist of Amino Acid: 1-235 and predicts a molecular mass of 25.8 kDa.

Molecule Mass

25.8 kDa

Verified

HPLC

Sequence

Amino Acid: 1-235

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Interleukin 10 Receptor Subunit Alpha

Function

Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well (PubMed:16982608).

In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively (PubMed:12133952).

These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy (PubMed:26962683).

Biological Process

Cytokine-mediated signaling pathway Source: GO_Central
Negative regulation of autophagy Source: UniProtKB
Positive regulation of receptor signaling pathway via JAK-STAT Source: UniProtKB
Regulation of synapse organization Source: Ensembl
Response to lipopolysaccharide Source: Ensembl
Ubiquitin-dependent endocytosis Source: UniProtKB

Cellular Location

Cell membrane; Cytoplasm

Involvement in disease

Inflammatory bowel disease 28, autosomal recessive (IBD28):
A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.

Topology

Extracellular: 22-235
Helical: 236-256
Cytoplasmic: 257-578

PTM

Phosphorylated. Phosphorylation of the cytoplasmic tail induced STAT3 activation.
Ubiquitinated by BTRC; ubiquitination leads to endocytosis and subsequent degradation of IL10RA.

Grk, M., Miskovic, R., Jeremic, I., Basaric, M., Dusanovic Pjevic, M., Jekic, B., ... & Banko, A. (2023). Association of IL10RA, IL10RB, and IL22RA Polymorphisms/Haplotypes with Susceptibility to and Clinical Manifestations of SLE. International Journal of Molecular Sciences, 24(14), 11292.

Mario-Vásquez, J. E., Naranjo-González, C. A., Montiel, J., Zuluaga, L. M., Vásquez, A. M., Tobón-Castaño, A., ... & Segura, C. (2021). Association of variants in IL1B, TLR9, TREM1, IL10RA, and CD3G and Native American ancestry on malaria susceptibility in Colombian populations. Infection, Genetics and Evolution, 87, 104675.

Tang, Z., Zhang, P., Ji, M., Yin, C., Zhao, R., Huang, Z., & Huang, Y. (2021). Characterization of novel and large fragment deletions in exon 1 of the IL10RA gene in Chinese children with very early onset inflammatory bowel diseases. BMC gastroenterology, 21(1), 1-8.

Cheng, S., Li, Z., Zhang, W., Sun, Z., Fan, Z., Luo, J., & Liu, H. (2021). Identification of IL10RA by weighted correlation network analysis and in vitro validation of its association with prognosis of metastatic melanoma. Frontiers in Cell and Developmental Biology, 8, 630790.

Prokoph, N., Probst, N. A., Lee, L. C., Monahan, J. M., Matthews, J. D., Liang, H. C., ... & Turner, S. D. (2020). IL10RA modulates crizotinib sensitivity in NPM1-ALK+ anaplastic large cell lymphoma. Blood, The Journal of the American Society of Hematology, 136(14), 1657-1669.

Xue, A. J., Miao, S. J., Sun, H., Qiu, X. X., Wang, S. N., Wang, L., ... & Huang, Y. (2020). Intestinal dysbiosis in pediatric Crohn's disease patients with IL10RA mutations. World journal of gastroenterology, 26(22), 3098.

Al-Abbasi, F. A., Mohammed, K., Sadath, S., Banaganapalli, B., Nasser, K., & Shaik, N. A. (2018). Computational protein phenotype characterization of IL10RA mutations causative to early onset inflammatory bowel disease (IBD). Frontiers in Genetics, 9, 146.

Peng, K., Qian, X., Huang, Z., Lu, J., Wang, Y., Zhou, Y., ... & Huang, Y. (2018). Umbilical cord blood transplantation corrects very early-onset inflammatory bowel disease in Chinese patients with IL10RA-associated immune deficiency. Inflammatory Bowel Diseases, 24(7), 1416-1427.

Zadka, Ł., Kulus, M. J., Kurnol, K., Piotrowska, A., Glatzel-Plucińska, N., Jurek, T., ... & Dzięgiel, P. (2018). The expression of IL10RA in colorectal cancer and its correlation with the proliferation index and the clinical stage of the disease. Cytokine, 110, 116-125.

Jung, E. S., Petersen, B. S., Mayr, G., Cheon, J. H., Kang, Y., Lee, S. J., ... & Koh, H. (2018). Compound heterozygous mutations in IL10RA combined with a complement factor properdin mutation in infantile-onset inflammatory bowel disease. European Journal of Gastroenterology & Hepatology, 30(12), 1491-1496.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant IL10RA protein, His Tag-1 (CAT#: V2LY-0526-LY4715)

Mouse Recombinant IL10RA protein, ECD, hFc Tag (CAT#: V2LY-0526-LY8459)

Rhesus Recombinant IL10RA protein, hFc Tag (CAT#: V2LY-0526-LY9733)

Rhesus Recombinant IL10RA protein, His Tag (CAT#: V2LY-0526-LY9734)

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Isotype control

For research use only. Not intended for any clinical use.

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