Human Recombinant IMPA1 protein, His Tag (V2LY-0526-LY4993)

Name: Human Recombinant IMPA1 protein, His Tag
SKU: V2LY-0526-LY4993
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant IMPA1 protein, His Tag consist of Amino Acid: 1-277 and predicts a molecular mass of 32.4 kDa.

Molecule Mass

32.4 kDa

Sequence

Amino Acid: 1-277

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, Glycerol, DTT

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Inositol Monophosphatase 1

Function

Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Has broad substrate specificity and can use myo-inositol monophosphates, myo-inositol 1,3-diphosphate, myo-inositol 1,4-diphosphate, scyllo-inositol-phosphate, D-galactose 1-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates.

Biological Process

Inositol biosynthetic processIEA:UniProtKB-UniPathway
Inositol metabolic processManual Assertion Based On ExperimentIBA:GO_Central
Inositol phosphate dephosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Phosphate-containing compound metabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Phosphatidylinositol biosynthetic processManual Assertion Based On ExperimentIMP:UniProtKB
Phosphatidylinositol phosphate biosynthetic processIEA:InterPro
Signal transductionManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Cytoplasm

Involvement in disease

Mental retardation, autosomal recessive 59 (MRT59):
A form of mental retardation, a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. MRT59 transmission pattern is consistent with autosomal recessive inheritance.

Yang, S. Y., Xie, Y. F., Zhang, T. M., Deng, L., Liao, L., Hu, S. Y., ... & Li, D. Q. (2023). Inositol monophosphatase 1 (IMPA1) promotes triple‐negative breast cancer progression through regulating mTOR pathway and EMT process. Cancer Medicine, 12(2), 1602-1615.

Pessoa, A. L. S., Quesada, A. A., Nóbrega, P. R., Viana, A. P. O., de Oliveira, K. T., Figueiredo, T., ... & Kok, F. (2023). Neuropsychological Characterization of Autosomal Recessive Intellectual Developmental Disorder 59 Associated with IMPA1 (MRT59). Brain Sciences, 13(7), 1048.

Padinjat, R., Saha, S., & Krishnan, H. (2022). IMPA1 dependent regulation of plasma membrane phosphatidylinositol 4, 5-bisphosphate turnover and calcium signalling by lithium. bioRxiv, 2022-10.

Andreassi, C., Luisier, R., Crerar, H., Darsinou, M., Blokzijl-Franke, S., Lenn, T., ... & Riccio, A. (2021). Cytoplasmic cleavage of IMPA1 3′ UTR is necessary for maintaining axon integrity. Cell Reports, 34(8).

Figueiredo, T., Mendes, A. P., Moreira, D. P., Goulart, E., Oliveira, D., Kobayashi, G. S., ... & Zatz, M. (2021). Inositol monophosphatase 1 (IMPA1) mutation in intellectual disability patients impairs neurogenesis but not gliogenesis. Molecular Psychiatry, 26(7), 3558-3571.

Pillai, R. A., Islam, M. O., Selvam, P., Sharma, N., Chu, A. H., Watkins, O. C., ... & Chan, S. Y. (2021). Placental inositol reduced in gestational diabetes as glucose alters inositol transporters and IMPA1 enzyme expression. The Journal of Clinical Endocrinology & Metabolism, 106(2), e875-e890.

Bogatikov, E., Lindblad, I., Punga, T., & Punga, A. R. (2020). miR-1933-3p is upregulated in skeletal muscles of MuSK+ EAMG mice and affects Impa1 and Mrpl27. Neuroscience research, 151, 46-52.

Walker, C. P., Pessoa, A. L., Figueiredo, T., Rafferty, M., Melo, U. S., Nóbrega, P. R., ... & Cho, R. Y. (2019). Loss-of-function mutation in inositol monophosphatase 1 (IMPA1) results in abnormal synchrony in resting-state EEG. Orphanet Journal of Rare Diseases, 14(1), 1-10.

Rosette, C., & Murray PhD, M. (2018). P-29 Preparing to Test the Effects of Omega-3 Fatty Acids on Inositol Levels and ISYNA & IMPA1 Gene Expression in Mammalian Cells.

de Farias, A. A., Nunes, K., Lemes, R. B., Moura, R., Fernandes, G. R., Melo, U. S., ... & Santos, S. (2018). Origin and age of the causative mutations in KLC2, IMPA1, MED25 and WNT7A unravelled through Brazilian admixed populations. Scientific reports, 8(1), 16552.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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