Human Recombinant ITGA2B (I874S) protein, Hetero Dimer , His Tag (V2LY-0526-LY199)

Name: Human Recombinant ITGA2B (I874S) protein, Hetero Dimer , His Tag
SKU: V2LY-0526-LY199
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant ITGA2B (I874S) protein, Hetero Dimer , His Tag consist of Amino Acid: 1-993, 1-718 and predicts a molecular mass of 191.12 kDa.

Molecule Mass

191.12 kDa

Polymeric

Hetero Dimer

Sequence

Amino Acid: 1-993, 1-718

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Integrin Subunit Alpha 2b

Alternative Names

Integrin Subunit Alpha 2b

Function

Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.

Biological Process

Cell-matrix adhesionIEA:Ensembl
Integrin-mediated signaling pathwayManual Assertion Based On ExperimentHDA:UniProtKB

Cellular Location

Membrane

Involvement in disease

Glanzmann thrombasthenia 1 (GT1):
A form of Glanzmann thrombasthenia, a disorder characterized by failure of platelet aggregation, absent or diminished clot retraction, and mucocutaneous bleeding of mild-to-moderate severity. Glanzmann thrombasthenia has been classified into clinical types I and II. In type I, platelets show absence of glycoprotein IIb-IIIa complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express glycoprotein IIb-IIIa complexes at reduced levels, have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. GT1 inheritance is autosomal recessive.
Bleeding disorder, platelet-type, 16 (BDPLT16):
An autosomal dominant form of congenital macrothrombocytopenia associated with platelet anisocytosis. It is a disorder of platelet production. Affected individuals may have no or only mildly increased bleeding tendency. In vitro studies show mild platelet functional abnormalities.

Topology

Extracellular: 32-993
Helical: 994-1019
Cytoplasmic: 1020-1039

Jiang, J., Li, W., Zhou, L., Liu, D., Wang, Y., An, J., ... & Xie, Z. (2023). Platelet ITGA2B inhibits caspase-8 and Rip3/Mlkl-dependent platelet death though PTPN6 during sepsis. Iscience, 26(8).

Zhao, J., Zhou, M., Wang, Z., Wu, L., Hu, Z., & Liang, D. (2022). Ectopic expression of FVIII in HPCs and MSCs derived from hiPSCs with site-specific integration of ITGA2B promoter-driven BDDF8 gene in hemophilia a. International Journal of Molecular Sciences, 23(2), 623.

Xie, Z., Jiang, J., Cao, L., Jiang, M., Yang, F., Ma, Z., ... & Zhou, L. (2021). Nonsense‐mediated mRNA decay efficiency influences bleeding severity in ITGA2B c. 2659C> T (p. Q887X) knock‐in mice. Clinical Genetics, 100(2), 213-218.

Ross, J. E., Zhang, B. M., Lee, K., Mohan, S., Branchford, B. R., Bray, P., ... & Di Paola, J. (2021). Specifications of the variant curation guidelines for ITGA2B/ITGB3: ClinGen platelet disorder variant curation panel. Blood Advances, 5(2), 414-431.

Karaman, K., Yürektürk, E., Geylan, H., Yaşar, A. Ş., Karaman, S., Aymelek, H. S., ... & Oner, A. F. (2021). Identification of three novel pathogenic ITGA2B and one novel pathogenic ITGB3 mutations in patients with hereditary Glanzmann’s thrombasthenia living in Eastern Turkey. Platelets, 32(2), 238-242.

Negahdari, S., Zamani, M., Seifi, T., Sedighzadeh, S., Mazaheri, N., Zeighami, J., ... & Galehdari, H. (2020). Identification of three novel mutations in the FANCA, FANCC, and ITGA2B genes by whole exome sequencing. International journal of preventive medicine, 11.

Li, R. H., Ontiveros, E., Nguyen, N., Stern, J. A., Lee, E., Hardy, B. T., & 99 Lives Cat Genome Consortium. (2020). Precision medicine identifies a pathogenic variant of the ITGA2B gene responsible for Glanzmann's thrombasthenia in a cat. Journal of veterinary internal medicine, 34(6), 2438-2446.

Xing, S., Zeng, T., Xue, N., He, Y., Lai, Y. Z., Li, H. L., ... & Liu, W. L. (2019). Development and validation of tumor-educated blood platelets integrin alpha 2b (ITGA2B) RNA for diagnosis and prognosis of non-small-cell lung cancer through RNA-seq. International journal of biological sciences, 15(9), 1977.

Khoriaty, R., Ozel, A. B., Ramdas, S., Ross, C., Desch, K., Shavit, J. A., ... & Ginsburg, D. (2019). Genome‐wide linkage analysis and whole‐exome sequencing identifies an ITGA 2B mutation in a family with thrombocytopenia. British journal of haematology, 186(4), 574-579.

Nurden, A. T., & Pillois, X. (2018). ITGA2B and ITGB3 gene mutations associated with Glanzmann thrombasthenia. Platelets, 29(1), 98-101.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant ITGA2B (R887L) protein, Hetero Dimer , His & AVI Tag (CAT#: V2LY-0526-LY200)

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For research use only. Not intended for any clinical use.

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