Human Recombinant MAP4K2, Active protein, GST Tag (V2LY-0526-LY4288)

Name: Human Recombinant MAP4K2, Active protein, GST Tag
SKU: V2LY-0526-LY4288
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant MAP4K2, Active protein, GST Tag consist of Amino Acid: Full Length and predicts a molecular mass of 125 kDa.

Molecule Mass

125 kDa

Sequence

Amino Acid: Full Length

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE KINASE 2

Function

Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extent of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation. The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3. May play a role in the regulation of vesicle targeting or fusion. regulation of vesicle targeting or fusion.

Biological Process

Immune responseManual Assertion Based On ExperimentTAS:ProtInc
Innate immune responseIEA:UniProtKB-KW
Intracellular signal transductionManual Assertion Based On ExperimentIDA:UniProtKB
JNK cascadeManual Assertion Based On ExperimentTAS:ProtInc
Positive regulation of JNK cascadeManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of JUN kinase activityManual Assertion Based On ExperimentIDA:UniProtKB
Protein phosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Vesicle targetingIEA:Ensembl

Cellular Location

Cytoplasm
Basolateral cell membrane
Golgi apparatus membrane

PTM

Polyubiquitinated through 'Lys-48'-polyubiquitin chains, allowing proteasomal turnover. Ubiquitination requires the kinase activity of MAP4K2/GCK.
Autophosphorylated in response to tumor necrosis factor (TNF), endotoxins or pro-inflammatory stimuli. Autophosphorylation leads to activation.

Seo, G., Mckinley, J., & Wang, W. (2023). MAP4K2 connects the Hippo pathway to autophagy in response to energy stress. Autophagy, 1-3.

Ma, C., Shi, Z. H., Han, X. Y., Liu, C., Yan, B., & Du, J. L. (2022). Targeting circRNA-MAP4K2 for the treatment of diabetes-induced retinal vascular dysfunction. Aging (Albany NY), 14(15), 6255.

Li, S., Fu, J., Yang, J., Ma, H., Mapara, M. Y., Marcireau, C., & Lentzsch, S. (2021). MAP4K2 Silencing Overcomes IMiDs-Resistance in Multiple Myeloma. Blood, 138, 2662.

Li, S., Fu, J., Xu, X., Deng, S., Landry, D., Mapara, M., & Lentzsch, S. (2019). Treatment of RAS-Mutated Multiple Myeloma by Targeting MAP4K2. Clinical Lymphoma, Myeloma and Leukemia, 19(10), e131-e132.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant MAP4K2 protein, GST Tag (CAT#: V2LY-0526-LY4289)

Related Products

Human Recombinant MAP4K2 protein, GST Tag (CAT#: V2LY-0526-LY4289)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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