Human Recombinant MAPKAPK3 protein, GST Tag-1 (V2LY-0526-LY5461)

Name: Human Recombinant MAPKAPK3 protein, GST Tag-1
SKU: V2LY-0526-LY5461
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant MAPKAPK3 protein, GST Tag consist of Amino Acid: 1-382 and predicts a molecular mass of 69 kDa.

Molecule Mass

69 kDa

Sequence

Amino Acid: 1-382

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Mitogen-Activated Protein Kinase-Activated Protein Kinase 3

Function

Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression.

Biological Process

Intracellular signal transductionManual Assertion Based On ExperimentIBA:GO_Central
MacropinocytosisISS:UniProtKB
Peptidyl-serine phosphorylationManual Assertion Based On ExperimentIDA:BHF-UCL
Protein autophosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Response to cytokineManual Assertion Based On ExperimentIDA:UniProtKB
Response to lipopolysaccharideISS:UniProtKB
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc
Toll-like receptor signaling pathwayISS:UniProtKB
Vascular endothelial growth factor receptor signaling pathwayTAS:Reactome

Cellular Location

Nucleus
Cytoplasm
Predominantly located in the nucleus, when activated it translocates to the cytoplasm.

Involvement in disease

Macular dystrophy, patterned, 3 (MDPT3):
A form of retinal patterned dystrophy, characterized by retinal pigment epithelium and Bruch's membrane changes resembling a 'dry desert land'. It begins around the age of 30 and progresses to retinitis pigmentosa. MDPT3 inheritance is autosomal dominant.

PTM

Phosphorylated and activated by MAPK1/ERK2 and MAPK3/ERK1. Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-201, Ser-251 and Thr-313 (By similarity).

Jickling, G., Sharp, F., Jauch, E., & BASE Investigators. (2020). Abstract TMP100: Biology of Stroke: Role of ELL2, GLIPR1, MAPKAPK3 Genes in Identifying Atrial Fibrillation Cause of Stroke. Stroke, 51(Suppl_1), ATMP100-ATMP100.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant MAPKAPK3 protein, GST Tag (CAT#: V2LY-0526-LY5459)

Human Recombinant MAPKAPK3, Active protein, GST Tag (CAT#: V2LY-0526-LY5460)

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Isotype control

For research use only. Not intended for any clinical use.

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