Human Recombinant MST1R protein, His Tag (V2LY-0526-LY6579)

Name: Human Recombinant MST1R protein, His Tag
SKU: V2LY-0526-LY6579
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant MST1R protein, His Tag consist of Amino Acid: 1-571 and predicts a molecular mass of 60 kDa.

Molecule Mass

60 kDa

Verified

HPLC

Sequence

Amino Acid: 1-571

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥98% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Macrophage Stimulating 1 Receptor

Function

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Plays also a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand.

Biological Process

Cell migration Source: GO_Central
Defense response Source: ProtInc
Innate immune response Source: UniProtKB-KW
Nervous system development Source: GO_Central
Phagocytosis Source: GO_Central
Positive regulation of cell population proliferation Source: ProtInc
Positive regulation of kinase activity Source: GO_Central
Positive regulation of MAP kinase activity Source: UniProtKB
Positive regulation of protein kinase B signaling Source: UniProtKB
Response to virus Source: UniProtKB
Signal transduction Source: ProtInc
Single fertilization Source: ProtInc
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central

Cellular Location

Membrane

Involvement in disease

Nasopharyngeal carcinoma, 3 (NPCA3):
A form of nasopharyngeal carcinoma, a malignant neoplasm that originates in the nasopharyngeal epithelium and includes 4 subtypes: keratinizing squamous cell, non-keratinizing, basaloid squamous cell, and papillary adenocarcinoma.

Topology

Extracellular: 25-957
Helical: 958-978
Cytoplasmic: 979-1400

PTM

Proteolytic processing yields the two subunits.
Autophosphorylated in response to ligand binding on Tyr-1238 and Tyr-1239 in the kinase domain leading to further phosphorylation of Tyr-1353 and Tyr-1360 in the C-terminal multifunctional docking site.
Ubiquitinated. Ubiquitination by CBL regulates the receptor stability and activity through proteasomal degradation.

Kim, J., Koh, D. I., Lee, M., Park, Y. S., Hong, S. W., Shin, J. S., ... & Jin, D. H. (2023). Targeting isoforms of RON kinase (MST1R) drives antitumor efficacy. Cell Death & Differentiation, 30(12), 2491-2507.

Hunt, B. G., Jones, A., Lester, C., Davis, J. C., Benight, N. M., & Waltz, S. E. (2022). RON (MST1R) and HGFL (MST1) co-overexpression supports breast tumorigenesis through autocrine and paracrine cellular crosstalk. Cancers, 14(10), 2493.

Qiu, S., Hu, Y., Zou, Q., & Liu, G. (2022). Genetic variant rs9848497 up-regulates MST1R expression, thereby influencing leadership phenotypes. Proceedings of the National Academy of Sciences, 119(29), e2207847119.

Robert, C., Marquevielle, J., & Salgado, G. F. (2022). The promoter region of the proto-oncogene MST1R contains the main features of G-quadruplexes formation. International Journal of Molecular Sciences, 23(21), 12905.

Cazes, A., Childers, B. G., Esparza, E., & Lowy, A. M. (2022). The MST1R/RON tyrosine kinase in cancer: oncogenic functions and therapeutic strategies. Cancers, 14(8), 2037.

Zhou, D., Zhu, X., Wu, X., Zheng, J., Tou, L., & Zhou, Y. (2021). The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR. Experimental and Therapeutic Medicine, 22(2), 1-9.

Yoon, J. Y., Wang, J. Y., & Roehrl, M. H. (2020). A combined FAK, c-MET, and MST1R three-protein panel risk-stratifies colorectal cancer patients. Translational Oncology, 13(11), 100836.

Hunt, B. G., Wicker, C. A., Bourn, J. R., Lower, E. E., Takiar, V., & Waltz, S. E. (2020). MST1R (RON) expression is a novel prognostic biomarker for metastatic progression in breast cancer patients. Breast cancer research and treatment, 181, 529-540.

Gupta, A., Yadav, S., Pt, A., Mishra, J., Samaiya, A., Panday, R. K., & Shukla, S. (2020). The HNRNPA2B1–MST1R–Akt axis contributes to epithelial-to-mesenchymal transition in head and neck cancer. Laboratory Investigation, 100(12), 1589-1601.

Babicky, M. L., Harper, M. M., Chakedis, J., Cazes, A., Mose, E. S., Jaquish, D. V., ... & Lowy, A. M. (2019). MST1R kinase accelerates pancreatic cancer progression via effects on both epithelial cells and macrophages. Oncogene, 38(28), 5599-5611.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant MST1R, Active protein, GST Tag (CAT#: V2LY-0526-LY6578)

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Isotype control

For research use only. Not intended for any clinical use.

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