Human Recombinant NT5E protein, hFc Tag (V2LY-0526-LY1867)

Name: Human Recombinant NT5E protein, hFc Tag
SKU: V2LY-0526-LY1867
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant NT5E protein, hFc Tag consist of Amino Acid: 1-547(T376A) and predicts a molecular mass of 84.5 kDa.

Molecule Mass

84.5 kDa

Sequence

Amino Acid: 1-547(T376A)

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

5'-Nucleotidase Ecto

Function

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

Biological Process

Adenosine biosynthetic processIEA:Ensembl
ADP catabolic processIEA:Ensembl
AMP catabolic processManual Assertion Based On ExperimentIBA:GO_Central
ATP metabolic processManual Assertion Based On ExperimentIDA:MGI
BiomineralizationManual Assertion Based On ExperimentIDA:MGI
Calcium ion homeostasisIEA:Ensembl
DNA metabolic processManual Assertion Based On ExperimentTAS:ProtInc
Leukocyte cell-cell adhesionManual Assertion Based On ExperimentIDA:CACAO
Negative regulation of inflammatory responseIEA:Ensembl
Response to ATPManual Assertion Based On ExperimentIDA:MGI
Response to inorganic substanceManual Assertion Based On ExperimentIDA:MGI

Cellular Location

Cell membrane

Involvement in disease

Calcification of joints and arteries (CALJA):
A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.

Barge, S., Wu, A., Zhang, L., Robson, S. C., Olumi, A., Alper, S. L., ... & Yu, W. (2024). Role of ecto‐5′‐nucleotidase in bladder function. The FASEB Journal, 38(2), e23416.

Kordaß, T., Chao, T. Y., Osen, W., & Eichmüller, S. B. (2023). Novel microRNAs modulating ecto-5′-nucleotidase expression. Frontiers in Immunology, 14, 1199374.

Murakami, Y., Ando, M., Futamata, R., Horibe, T., Ueda, K., Kinoshita, M., & Kobayashi, T. (2022). Targeted deletion of ecto-5′-nucleotidase results in retention of inosine monophosphate content in postmortem muscle of medaka (Oryzias latipes). Scientific Reports, 12(1), 18588.

da Silva, F. C., de Melo Neto, A. B., Martins, C. A., de Sousa Cardoso, T. C., de Souza Gomes, M., de Araújo, T. G., & Fürstenau, C. R. (2022). Is the regulation by miRNAs of NTPDase1 and ecto-5’-nucleotidase genes involved with the different profiles of breast cancer subtypes?. Purinergic Signalling, 18(1), 123-133.

Alcedo, K. P., Bowser, J. L., & Snider, N. T. (2021). The elegant complexity of mammalian ecto-5′-nucleotidase (CD73). Trends in cell biology, 31(10), 829-842.

Shali, S., Yu, J., Zhang, X., Wang, X., Jin, Y., Su, M., ... & Zhou, P. (2019). Ecto‐5′‐nucleotidase (CD73) is a potential target of hepatocellular carcinoma. Journal of cellular physiology, 234(7), 10248-10259.

Minor, M., Alcedo, K. P., Battaglia, R. A., & Snider, N. T. (2019). Cell type-and tissue-specific functions of ecto-5′-nucleotidase (CD73). American Journal of Physiology-Cell Physiology, 317(6), C1079-C1092.

Hu, S., Meng, F., Yin, X., Cao, C., & Zhang, G. (2019). NT5E is associated with unfavorable prognosis and regulates cell proliferation and motility in gastric cancer. Bioscience reports, 39(5), BSR20190101.

Bertoni, A. P. S., Bracco, P. A., de Campos, R. P., Lutz, B. S., Assis-Brasil, B. M., de Souza Meyer, E. L., ... & Wink, M. R. (2019). Activity of ecto-5′-nucleotidase (NT5E/CD73) is increased in papillary thyroid carcinoma and its expression is associated with metastatic lymph nodes. Molecular and cellular endocrinology, 479, 54-60.

Kobarfard, F., Noroozi, R., Beirami, A. D., Taheri, M., & Ghafouri-Fard, S. (2019). Dys-regulation of peripheral transcript levels of ecto-5’-nucleotidase in multiple sclerosis patients. Human Antibodies, 27(3), 161-165.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant NT5E protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY1868)

Human Recombinant NT5E protein, His Tag (CAT#: V2LY-0526-LY1869)

Rat Recombinant NT5E protein, His Tag (CAT#: V2LY-0526-LY9150)

Cynomolgus Recombinant NT5E protein, His Tag (CAT#: V2LY-0526-LY1363)

Mouse Recombinant NT5E protein, His Tag (CAT#: V2LY-0526-LY7760)

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Isotype control

For research use only. Not intended for any clinical use.

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