Human Recombinant OSTM1 protein, His Tag (V2LY-0526-LY5891)

Name: Human Recombinant OSTM1 protein, His Tag
SKU: V2LY-0526-LY5891
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant OSTM1 protein, His Tag consist of Amino Acid: 1-284 and predicts a molecular mass of 29.7 kDa.

Molecule Mass

29.7 kDa

Sequence

Amino Acid: 1-284

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>97% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile MES, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

osteopetrosis associated transmembrane protein 1

Function

Required for osteoclast and melanocyte maturation and function.

Biological Process

Osteoclast differentiationManual Assertion Based On ExperimentIBA:GO_Central
Transepithelial chloride transportManual Assertion Based On ExperimentIDA:ComplexPortal

Cellular Location

Lysosome membrane
Requires CLCN7 to travel to lysosomes.

Involvement in disease

Osteopetrosis, autosomal recessive 5 (OPTB5):
A rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. Osteopetrosis occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Recessive osteopetrosis commonly manifests in early infancy with macrocephaly, feeding difficulties, evolving blindness and deafness, bone marrow failure, severe anemia, and hepatosplenomegaly. Deafness and blindness are generally thought to represent effects of pressure on nerves. OPTB5 patients manifest primary central nervous system involvement in addition to the classical stigmata of severe bone sclerosis, growth failure, anemia, thrombocytopenia and visual impairment with optic atrophy.

Topology

Lumenal: 32-284
Helical: 285-305
Cytoplasmic: 306-334

PTM

Undergoes proteolytic cleavage in the luminal domain, the cleaved fragments might be linked by disulfide bonds with the remnant of the protein.
Highly N-glycosylated.

Alotaibi, Q., Dighe, M., & Aldaihani, S. (2023). The clinical features of OSTM1‐associated malignant infantile osteopetrosis: A retrospective, single‐center experience over one decade. American Journal of Medical Genetics Part A, 191(2), 459-468.

Vacher, J. (2022). OSTM1 pleiotropic roles from osteopetrosis to neurodegeneration. Bone, 163, 116505.

Du, J., Xiao, J., Zhuo, Y., Nie, M., Yang, J., Luo, X., & Gu, H. (2022). LncRNA OSTM1-AS1 acts as an oncogenic factor in Wilms’ tumor by regulating the miR-514a-3p/MELK axis. Anti-Cancer Drugs, 33(8), 720-730.

Alotaibi, Q., & Dighe, M. (2021). Managing challenging pain and irritability in OSTM1 mutation-related infantile malignant osteopetrosis. BMJ Case Reports CP, 14(5), e242498.

Pata, M., Yousefi Behzadi, P., & Vacher, J. (2021). Expression pattern of the V5‐Ostm1 protein in bacterial artificial chromosome transgenic mice. genesis, 59(3), e23409.

Zhang, S., Liu, Y., Zhang, B., Zhou, J., Li, T., Liu, Z., ... & Yang, M. (2020). Molecular insights into the human CLC-7/Ostm1 transporter. Science Advances, 6(33), eabb4747.

Schrecker, M., Korobenko, J., & Hite, R. K. (2020). Cryo-EM structure of the lysosomal chloride-proton exchanger CLC-7 in complex with OSTM1. Elife, 9, e59555.

Vacher, J., Bruccoleri, M., & Pata, M. (2020). Ostm1 from mouse to human: Insights into osteoclast maturation. International Journal of Molecular Sciences, 21(16), 5600.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant OSTM1 protein, His Tag (CAT#: V2LY-0526-LY8730)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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