Human Recombinant STIM1 protein, His Tag (V2LY-0526-LY6986)

Name: Human Recombinant STIM1 protein, His Tag
SKU: V2LY-0526-LY6986
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant STIM1 protein, His Tag consist of Amino Acid: 1-213 and predicts a molecular mass of 23.3 kDa.

Molecule Mass

23.3 kDa

Sequence

Amino Acid: 1-213

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>97% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

stromal interaction molecule 1

Function

Plays a role in mediating store-operated Ca2+ entry (SOCE), a Ca2+ influx following depletion of intracellular Ca2+ stores (PubMed:15866891, PubMed:16005298, PubMed:16208375, PubMed:16537481, PubMed:16733527, PubMed:16766533, PubMed:16807233, PubMed:18854159, PubMed:19249086, PubMed:22464749, PubMed:24069340, PubMed:24351972, PubMed:24591628, PubMed:26322679, PubMed:25326555, PubMed:28219928).
Acts as Ca2+ sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca2+ depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca2+ release-activated Ca2+ (CRAC) channel subunit ORAI1 (PubMed:16208375, PubMed:16537481).
Involved in enamel formation (PubMed:24621671).
Activated following interaction with STIMATE, leading to promote STIM1 conformational switch (PubMed:26322679).

Biological Process

Biological Process activation of store-operated calcium channel activityManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process cellular calcium ion homeostasisManual Assertion Based On ExperimentIBA:GO_Central
Biological Process detection of calcium ionManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process enamel mineralizationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of adenylate cyclase activityManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of angiogenesisManual Assertion Based On ExperimentIMP:BHF-UCL
Biological Process regulation of calcium ion transportManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of store-operated calcium entryManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process store-operated calcium entryManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Cell membrane
Endoplasmic reticulum membrane
Cytoplasm, cytoskeleton
Sarcoplasmic reticulum
Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium and is detected at punctae corresponding to junctions between the endoplasmic reticulum and the cell membrane (PubMed:19249086, PubMed:16005298, PubMed:16208375, PubMed:18854159).
Associated with the microtubule network at the growing distal tip of microtubules (PubMed:19632184).
Colocalizes with ORAI1 at the cell membrane (PubMed:27185316).
Colocalizes preferentially with CASQ1 at endoplasmic reticulum in response to a depletion of intracellular calcium (PubMed:27185316).

Involvement in disease

Immunodeficiency 10 (IMD10):
An immune disorder characterized by recurrent infections, impaired activation and proliferative response of T-cells, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels. Additional features include thrombocytopenia, autoimmune hemolytic anemia, myopathy, partial iris hypoplasia, hepatosplenomegaly and defective enamel dentition.
Myopathy, tubular aggregate, 1 (TAM1):
A rare congenital myopathy characterized by regular arrays of membrane tubules on muscle biopsies without additional histopathological hallmarks. Tubular aggregates in muscle are structures of variable appearance consisting of an outer tubule containing either one or more microtubule-like structures or amorphous material. They may occur in a variety of circumstances, including inherited myopathies, alcohol- and drug-induced myopathies, exercise-induced cramps or muscle weakness.
Stormorken syndrome (STRMK):
A rare autosomal dominant disease characterized by mild bleeding tendency, thrombocytopathy, thrombocytopenia, mild anemia, asplenia, tubular aggregate myopathy, miosis, headache, and ichthyosis.

Topology

Extracellular: 23-213
Helical: 214-234
Cytoplasmic: 235-685

PTM

Glycosylation is required for cell surface expression.
Phosphorylated predominantly on Ser residues.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human STIM1 ELISA Kit (CAT#: V2LY-0626-LY1797)

Rat Stim1 ELISA Kit (CAT#: V2LY-0626-LY2684)

Human STIM1 ELISA Kit (2) (CAT#: V2LY-0626-LY5288)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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