Human Recombinant TAOK2, Active protein, GST Tag (V2LY-0526-LY7054)

TAO kinase 2

Serine/threonine-protein kinase involved in different processes such as membrane blebbing and apoptotic bodies formation DNA damage response and MAPK14/p38 MAPK stress-activated MAPK cascade. Phosphorylates itself, MBP, activated MAPK8, MAP2K3, MAP2K6 and tubulins. Activates the MAPK14/p38 MAPK signaling pathway through the specific activation and phosphorylation of the upstream MAP2K3 and MAP2K6 kinases. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Isoform 1, but not isoform 2, plays a role in apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation. This function, which requires the activation of MAPK8/JNK and nuclear localization of C-terminally truncated isoform 1, may be linked to the mitochondrial CASP9-associated death pathway. Isoform 1 binds to microtubules and affects their organization and stability independently of its kinase activity. Prevents MAP3K7-mediated activation of CHUK, and thus NF-kappa-B activation, but not that of MAPK8/JNK. May play a role in the osmotic stress-MAPK8 pathway. Isoform 2, but not isoform 1, is required for PCDH8 endocytosis. Following homophilic interactions between PCDH8 extracellular domains, isoform 2 phosphorylates and activates MAPK14/p38 MAPK which in turn phosphorylates isoform 2. This process leads to PCDH8 endocytosis and CDH2 cointernalization. Both isoforms are involved in MAPK14 phosphorylation.

Biological Process actin cytoskeleton organizationIDA:UniProtKB1 Publication
Biological Process activation of protein kinase activityTAS:ARUK-UCL1 Publication
Biological Process apoptotic processNAS:UniProtKB1 Publication
Biological Process axonogenesisIGI:ARUK-UCL1 Publication
Biological Process basal dendrite arborizationISS:ARUK-UCLBy Similarity
Biological Process basal dendrite morphogenesisISS:ARUK-UCLBy Similarity
Biological Process cell migrationNAS:UniProtKB1 Publication
Biological Process cellular response to DNA damage stimulusIDA:UniProtKB1 Publication
Biological Process focal adhesion assemblyIDA:UniProtKB1 Publication
Biological Process intracellular signal transductionIBA:GO_Central1 Publication
Biological Process MAPK cascadeIBA:GO_Central1 Publication
Biological Process mitotic G2 DNA damage checkpoint signalingIMP:UniProtKB1 Publication
Biological Process neuron projection morphogenesisIBA:GO_Central1 Publication
Biological Process positive regulation of JNK cascadeIDA:UniProtKB1 Publication
Biological Process positive regulation of MAPK cascadeIDA:UniProtKB1 Publication
Biological Process positive regulation of protein autophosphorylationTAS:ARUK-UCL1 Publication
Biological Process positive regulation of stress-activated MAPK cascadeIMP:UniProtKB1 Publication
Biological Process protein autophosphorylationTAS:ARUK-UCL1 Publication
Biological Process protein phosphorylationIBA:GO_Central1 Publication
Biological Process protein targeting to membraneNAS:UniProtKB1 Publication
Biological Process regulation of actin cytoskeleton organizationISS:ARUK-UCLBy Similarity
Biological Process regulation of cell growthNAS:UniProtKB1 Publication
Biological Process regulation of cell shapeIDA:UniProtKB1 Publication
Biological Process regulation of MAPK cascadeIBA:GO_Central1 Publication
Biological Process stress-activated MAPK cascadeIDA:UniProtKB1 Publication

Cytoplasmic vesicle membrane
Cytoplasm, cytoskeleton
Nucleus
Catalytically active full-length phosphorylated isoform 1 localizes to microtubules in the cytoplasm predominantly on microtubule cables positioned around the nucleus. A C-terminally truncated form of isoform 1 is present in the nucleus; isoform 2 and kinase-defective, as well as full-length isoform 1 are excluded from the nucleus.
Isoform 2
Cell projection, dendrite
In dendrites, colocalizes with PCDH8.

Isoforms 1 and 2 are autophosphorylated.
C-terminal cleavage of isoform 1 and subsequent nuclear localization requires CASP9 activity.
Autophosphorylated. Phosphorylated by ATM.
Isoform 2
Phosphorylated on Ser-1031 by MAPK14. This phosphorylation is required PCDH8 for endocytosis (By similarity).