Human Recombinant UBA6 protein, GST Tag (V2LY-0526-LY7476)

Name: Human Recombinant UBA6 protein, GST Tag
SKU: V2LY-0526-LY7476
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant UBA6 protein, GST Tag consist of Amino Acid: 1-1052 and predicts a molecular mass of 143.56 kDa.

Molecule Mass

143.56 kDa

Sequence

Amino Acid: 1-1052

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥80% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

ubiquitin-like modifier activating enzyme 6

Function

Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.

Biological Process

Biological Process amygdala development Source:Ensembl
Biological Process cellular response to DNA damage stimulus Source:GO_Central1 Publication
Biological Process dendritic spine development Source:Ensembl
Biological Process hippocampus development Source:Ensembl
Biological Process learning Source:Ensembl
Biological Process locomotory behavior Source:Ensembl
Biological Process protein modification by small protein conjugation Source:GO_Central1 Publication
Biological Process protein ubiquitination Source:UniProtKB1 Publication
Biological Process ubiquitin-dependent protein catabolic process Source:UniProtKB1 Publication

Cellular Location

cytoplasm
cytosol
nucleus

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant UBA6, Active protein, GST Tag (CAT#: V2LY-0526-LY7475)

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Mouse Anti-UBA6 Recombinant Antibody (CBFYU-033) (CAT#: CBMAB-U0033-FY)

Human Recombinant UBA6, Active protein, GST Tag (CAT#: V2LY-0526-LY7475)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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