Human Recombinant VRK2, Active protein, GST Tag (V2LY-0526-LY7650)

Vaccinia Related Kinase 2

Serine/threonine kinase that regulates several signal transduction pathways (PubMed:16704422, PubMed:14645249, PubMed:16495336, PubMed:17709393, PubMed:18617507, PubMed:18286207, PubMed:20679487).
Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes (PubMed:17709393).
Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription (PubMed:18286207).
Phosphorylates 'Thr-18' of p53/TP53, histone H3, and may also phosphorylate MAPK8IP1 (PubMed:16704422).
Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins (PubMed:16495336).
Down-regulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1 (PubMed:20679487).
Blocks the phosphorylation of ERK in response to ERBB2 and HRAS (PubMed:20679487).
Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant (PubMed:14645249).
Isoform 2
Phosphorylates 'Thr-18' of p53/TP53, as well as histone H3. Reduces p53/TP53 ubiquitination by MDM2, promotes p53/TP53 acetylation by EP300 and thereby increases p53/TP53 stability and activity.

Biological Process cellular response to oxidative stress Source:UniProtKB1 Publication
Biological Process peptidyl-serine phosphorylation Source:GO_Central1 Publication
Biological Process protein autophosphorylation Source:UniProtKB2 Publications
Biological Process protein phosphorylation Source:ProtInc1 Publication
Biological Process regulation of interleukin-1-mediated signaling pathway Source:UniProtKB1 Publication
Biological Process regulation of MAPK cascade Source:UniProtKB1 Publication
Biological Process signal transduction Source:GO_Central1 Publication

Isoform 1
Cytoplasm
Endoplasmic reticulum membrane
Mitochondrion membrane
Nucleus envelope
Isoform 2
Cytoplasm
Nucleus

Helical: 487-507

Isoform 1
Autophosphorylated.
Isoform 2
Autophosphorylated.