Human Recombinant VTI1A protein, His Tag (V2LY-0526-LY7660)

Name: Human Recombinant VTI1A protein, His Tag
SKU: V2LY-0526-LY7660
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant VTI1A protein, His Tag consist of Amino Acid: 1-187 and predicts a molecular mass of 24.2 kDa.

Molecule Mass

24.2 kDa

Sequence

Amino Acid: 1-187

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Vesicle Transport Through Interaction With T-SNAREs 1A

Function

V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non-conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. May be involved in increased cytokine secretion associated with cellular senescence.

Biological Process

Biological Process autophagy Source:ParkinsonsUK-UCL1 Publication
Biological Process endocytic recycling Source:UniProtKB1 Publication
Biological Process endoplasmic reticulum to Golgi vesicle-mediated transport Source:ParkinsonsUK-UCL
Biological Process Golgi to vacuole transport Source:GO_Central1 Publication
Biological Process intra-Golgi vesicle-mediated transport Source:GO_Central1 Publication
Biological Process intracellular protein transport Source:InterPro
Biological Process macroautophagy Source:GO_Central1 Publication
Biological Process retrograde transport, endosome to Golgi Source:UniProtKB1 Publication
Biological Process vesicle fusion Source:GO_Central1 Publication
Biological Process vesicle fusion with Golgi apparatus Source:ParkinsonsUK-UCL
Biological Process voluntary musculoskeletal movement Source:UniProtKB1 Publication

Cellular Location

Cytoplasmic vesicle
Golgi apparatus membrane

Topology

Cytoplasmic: 1-192
Helical: 193-213
Extracellular: 214-217

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant VTI1A protein, Fc Tag (CAT#: V2LY-0526-LY7661)

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Mouse Anti-VTI1A Recombinant Antibody (1F4) (CAT#: CBMAB-V0095-YC)

Human Recombinant VTI1A protein, Fc Tag (CAT#: V2LY-0526-LY7661)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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