Human Recombinant WDR48 protein, GST Tag (V2LY-0526-LY7664)

WD Repeat Domain 48

Regulator of deubiquitinating complexes, which acts as a strong activator of USP1, USP12 and USP46 (PubMed:18082604, PubMed:19075014, PubMed:31253762, PubMed:26388029).
Enhances the USP1-mediated deubiquitination of FANCD2; USP1 being almost inactive by itself (PubMed:18082604, PubMed:31253762).
Activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate (PubMed:19075014, PubMed:27373336).
Also activates deubiquitinating activity of complexes containing USP12 (PubMed:19075014, PubMed:27650958, PubMed:27373336).
In complex with USP12, acts as a potential tumor suppressor by positively regulating PHLPP1 stability (PubMed:24145035).
Docks at the distal end of the USP12 fingers domain and induces a cascade of structural changes leading to the activation of the enzyme (PubMed:27650958, PubMed:27373336).
Together with RAD51AP1, promotes DNA repair by stimulating RAD51-mediated homologous recombination (PubMed:27463890, PubMed:27239033, PubMed:32350107).
Binds single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) (PubMed:27239033, PubMed:31253762, PubMed:32350107).
DNA-binding is required both for USP1-mediated deubiquitination of FANCD2 and stimulation of RAD51-mediated homologous recombination: both WDR48/UAF1 and RAD51AP1 have coordinated role in DNA-binding during these processes (PubMed:31253762, PubMed:32350107).
Together with ATAD5 and by regulating USP1 activity, has a role in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA (PubMed:20147293).
Together with ATAD5, has a role in recruiting RAD51 to stalled forks during replication stress (PubMed:31844045).
(Microbial infection) In case of infection by Herpesvirus saimiri, may play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes. Induces lysosomal vesicle formation via interaction with Herpesvirus saimiri tyrosine kinase-interacting protein (TIP). Subsequently, TIP recruits tyrosine-protein kinase LCK, resulting in down-regulation of T-cell antigen receptor TCR. May play a role in generation of enlarged endosomal vesicles via interaction with TIP (PubMed:12196293).
In case of infection by papillomavirus HPV11, promotes the maintenance of the viral genome via its interaction with HPV11 helicase E1 (PubMed:18032488).

Biological Process cellular response to DNA damage stimulus Source:UniProtKB2 Publications
Biological Process double-strand break repair via homologous recombination Source:GO_Central1 Publication
Biological Process embryonic organ development Source:Ensembl
Biological Process homeostasis of number of cells Source:Ensembl
Biological Process multicellular organism growth Source:Ensembl
Biological Process positive regulation of double-strand break repair via homologous recombination Source:UniProtKB2 Publications
Biological Process positive regulation of epithelial cell proliferation Source:Ensembl
Biological Process protein deubiquitination Source:GO_Central1 Publication
Biological Process regulation of protein monoubiquitination Source:Ensembl
Biological Process seminiferous tubule development Source:Ensembl
Biological Process single fertilization Source:Ensembl
Biological Process skeletal system morphogenesis Source:Ensembl
Biological Process skin development Source:Ensembl
Biological Process spermatogenesis Source:Ensembl

Nucleus
Cytoplasm
Lysosome
Late endosome
Mainly in cytoplasmic compartments (PubMed:12196293, PubMed:18032488).
In case of infection by papillomavirus HPV11, translocates to the nucleus via its interaction with papillomavirus HPV11 (PubMed:18032488).