Rat Asl ELISA Kit (V2LY-0626-LY1543)

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Tested Data
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Basic Information

Sensitivity
0.16 ng/mL
Detection Range
0.3-90 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Rat
Assay Type
Sandwich
Reactivity
Rat
Assay Time
1.5 h
Molecule Mass
51.5 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
argininosuccinate lyase
Biological Process
Arginine biosynthetic process via ornithine Source: GO_Central
Urea cycle Source: Reactome
Cellular Location
Cytosol; Extracellular exosome; Cytoplasm
Involvement in disease
Argininosuccinic aciduria (ARGINSA): An autosomal recessive disorder of the urea cycle. The disease is characterized by mental and physical retardation, liver enlargement, skin lesions, dry and brittle hair showing trichorrhexis nodosa microscopically and fluorescing red, convulsions, and episodic unconsciousness.
PTM
Acetylation modifies enzyme activity in response to alterations of extracellular nutrient availability. Acetylation increased with trichostin A (TSA) or with nicotinamide (NAM). Glucose increases acetylation by about a factor of 3 with decreasing enzyme activity. Acetylation on Lys-288 is decreased on the addition of extra amino acids resulting in activation of enzyme activity.

Burrage, L. C., Madan, S., Li, X., Ali, S., Mohammad, M., Stroup, B. M., ... & Urea Cycle Disorders Consortium. (2020). Chronic liver disease and impaired hepatic glycogen metabolism in argininosuccinate lyase deficiency. JCI insight, 5(4).

Ali, E. Z., Yakob, Y., & Ngu, L. H. (2019). Identification of mutations in Malaysian patients with argininosuccinate lyase (ASL) deficiency. Molecular genetics and metabolism reports, 21, 100525.

Zhao, M., Hou, L., Teng, H., Li, J., Wang, J., Zhang, K., & Yang, L. (2019). Whole-Exome Sequencing Identified a Novel Compound Heterozygous Genotype in ASL in a Chinese Han Patient with Argininosuccinate Lyase Deficiency. BioMed research international, 2019.

Wang, Y., Sun, Y., Liu, M., Zhang, X., & Jiang, T. (2019). Functional characterization of argininosuccinate lyase gene variants by mini-gene splicing assay. Frontiers in genetics, 10, 436.

Kho, J., Tian, X., Wong, W. T., Bertin, T., Jiang, M. M., Chen, S., ... & Lee, B. H. (2018). Argininosuccinate lyase deficiency causes an endothelial-dependent form of hypertension. The American Journal of Human Genetics, 103(2), 276-287.

Kim, D., Ko, J. M., Kim, Y. M., Seo, G. H., Kim, G. H., Lee, B. H., & Yoo, H. W. (2018). Low prevalence of argininosuccinate lyase deficiency among inherited urea cycle disorders in Korea. Journal of human genetics, 63(8), 911-917.

Hung, Y. H., Huang, H. L., Chen, W. C., Yen, M. C., Cho, C. Y., Weng, T. Y., ... & Lai, M. D. (2017). Argininosuccinate lyase interacts with cyclin A2 in cytoplasm and modulates growth of liver tumor cells. Oncology reports, 37(2), 969-978.

Huang, H. L., Chen, W. C., Hsu, H. P., Cho, C. Y., Hung, Y. H., Wang, C. Y., & Lai, M. D. (2017). Silencing of argininosuccinate lyase inhibits colorectal cancer formation. Oncology reports, 37(1), 163-170.

Rostami, P., Häberle, J., Setoudeh, A., Zschocke, J., & Sayarifard, F. (2017). Two novel mutations in the argininosuccinate lyase gene in Iranian patients and literature review. Iranian Journal of Pediatrics, 27(3).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant ASL protein, GST Tag (CAT#: V2LY-0526-LY2167)

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Isotype control

For research use only. Not intended for any clinical use.

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