Human B4GALT1 ELISA Kit (V2LY-0626-LY5507)

Name: Human B4GALT1 ELISA Kit
SKU: V2LY-0626-LY5507
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.023 ng/mL

Detection Range

0.05-20 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

43.9 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Beta-1,4-Galactosyltransferase 1

Function

Beta-1,4-galactosyltransferase 1: The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
Processed beta-1,4-galactosyltransferase 1: The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.

Biological Process

Acute inflammatory response Source: Ensembl
Angiogenesis involved in wound healing Source: Ensembl
Binding of sperm to zona pellucida Source: Reactome
Cell adhesion Source: Ensembl
Development of secondary sexual characteristics Source: Ensembl
Epithelial cell development Source: Ensembl
Extracellular matrix organization Source: Ensembl
Galactose metabolic process Source: Ensembl
Glycosylation Source: GO_Central
Keratan sulfate biosynthetic process Source: Reactome
Lactose biosynthetic process Source: Reactome
Leukocyte migration Source: Ensembl
Negative regulation of cell population proliferation Source: Ensembl
neutrophil degranulation Source: Reactome
Oligosaccharide biosynthetic process Source: UniProtKB
Penetration of zona pellucida Source: Ensembl
Positive regulation of apoptotic process Source: Ensembl
Positive regulation of epithelial cell proliferation involved in wound healing Source: Ensembl
Protein N-linked glycosylation Source: UniProtKB
Regulation of acrosome reaction Source: Ensembl

Cellular Location

Isoform Long: Cell membrane; Golgi stack membrane; Cell surface; Filopodium. Found in trans cisternae of Golgi but is mainly localized at the plasma membrane (PubMed:1714903). B4GALT1 cell surface expression is regulated by UBE2Q1 (By similarity).
Isoform Short: Golgi stack membrane. Found in trans cisternae of Golgi.
Processed beta-1,4-galactosyltransferase 1: Secreted. Soluble form found in body fluids.

Involvement in disease

Congenital disorder of glycosylation 2D (CDG2D): A multisystem disorder caused by a defect in glycoprotein biosynthesis and characterized by under-glycosylated serum glycoproteins. Congenital disorders of glycosylation result in a wide variety of clinical features, such as defects in the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions.

Topology

Cytoplasmic: 1-24 aa
Helical: 25-44 aa
Lumenal: 45-398 aa

PTM

The soluble form derives from the membrane forms by proteolytic processing.

Chen, Y., Su, L., Huang, C., Wu, S., Qiu, X., Zhao, X., ... & Wong, P. P. (2021). Galactosyltransferase B4GALT1 confers chemoresistance in pancreatic ductal adenocarcinomas by upregulating N-linked glycosylation of CDK11p110. Cancer Letters, 500, 228-243.

Di Buduo, C. A., Giannini, S., Abbonante, V., Rosti, V., Hoffmeister, K. M., & Balduini, A. (2021). Increased B4GALT1 expression is associated with platelet surface galactosylation and thrombopoietin plasma levels in MPNs. Blood, 137(15), 2085-2089.

Valsalan, J., Sadan, T., Venkatachalapathy, T., Anilkumar, K., & Aravindakshan, T. V. (2020). Identification of novel single-nucleotide polymorphism at exon1 and 2 region of B4GALT1 gene and its association with milk production traits in crossbred cattle of Kerala, India. Animal Biotechnology, 1-9.

Montasser, M. E., Van Hout, C. V., McFarland, R., Rosenberg, A., Callaway, M., Shen, B., ... & Shuldiner, A. R. (2019). Genetic and functional evidence relates a missense variant in B4GALT1 to lower LDL-C and fibrinogen. bioRxiv, 721704.

Nilius, V., Killer, M. C., Timmesfeld, N., Schmitt, M., Moll, R., Lorch, A., ... & Brendel, C. (2018). High β-1, 4-Galactosyltransferase-I expression in peripheral T-lymphocytes is associated with a low risk of relapse in germ-cell cancer patients receiving high-dose chemotherapy with autologous stem cell reinfusion. Oncoimmunology, 7(5), e1423169.

Chen, P. D., Chang, A. A., Wu, Y. M., & Huang, M. C. (2018). Silencing of B4GALT1 inversely regulates malignant phenotypes of hepatocellular carcinoma cells.

Cipriano, V. T. F., Miranda-Furtado, C. L., Mioranza, A. A., de Paz, C. C. P., Vila, R. A., Ramos, E. S., & Lôbo, R. B. (2018). B4GALT1, LHR, FSHR and IGF2 polymorphisms and scrotal circumference in Nellore bulls. Revista Brasileira de Reprodução Animal, 42(2), 70-75.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant B4GALT1 protein, His Tag (CAT#: V2LY-0526-LY2236)

Human Recombinant B4GALT1 protein, His Tag-1 (CAT#: V2LY-0526-LY2237)

Mouse Recombinant B4GALT1 protein, His Tag (CAT#: V2LY-0526-LY7835)

Mouse B4galt1 ELISA Kit (CAT#: V2LY-0626-LY2073)

Cattle B4GALT1 ELISA Kit (CAT#: V2LY-0626-LY2481)

Related Products

Mouse Recombinant B4GALT1 protein, His Tag (CAT#: V2LY-0526-LY7835)

Human Recombinant B4GALT1 protein, His Tag-1 (CAT#: V2LY-0526-LY2237)

Mouse B4galt1 ELISA Kit (CAT#: V2LY-0626-LY2073)

Mouse Anti-B4GALT1 Recombinant Antibody (EG331) (CAT#: CBMAB-EN368-LY)

Cattle B4GALT1 ELISA Kit (CAT#: V2LY-0626-LY2481)

Human Recombinant B4GALT1 protein, His Tag (CAT#: V2LY-0526-LY2236)

Rabbit Anti-B4GALT1 Recombinant Antibody (CBYY-1652) (CAT#: CBMAB-2001-YY)

Mouse Anti-B4GALT1 Recombinant Antibody (CBYY-0090) (CAT#: CBMAB-0090-YY)

Mouse Anti-B4GALT1 Recombinant Antibody (CBYY-0092) (CAT#: CBMAB-0092-YY)

Mouse Anti-B4GALT1 Recombinant Antibody (CBYY-0091) (CAT#: CBMAB-0091-YY)

Isotype control

For research use only. Not intended for any clinical use.

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