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Human Immune Response Antibody Array (Glass Slide, 40 Targets) (Y0544) (AbAr-0544-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The product provided herein is a Human Immune Response Antibody Array, which can be used for semi-quantitative detection of 40 human immune response associated cytokines. It is suitable for a range of liquid forms including Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates.
See all Immune Response antibodies

Specifications

Target Array
Immune Response Array
Target
CD14, CD163, CD40(TNFRSF5), CRP(C-Reactive Protein), E-Selectin, Fas(TNFRSF6/Apo-1), Fas Ligand(TNFSF6), GCSF, ICAM-1(CD54), IL-1 alpha(IL-1 F1), IL-1 beta(IL-1 F2), IL-1 R4(ST2), IL-10, IL-12 p70, IL-13, IL-18, IL-2, IL-2 R alpha, IL-4, IL-6, IL-8(CXCL8), Lipocalin-2(NGAL), MCP-1(CCL2), MCP-2(CCL8), MIF, MIP-1 alpha(CCL3), MIP-1 beta(CCL4), Osteopontin(SPP1), PAI-1, Platelet Factor 4(CXCL4), Procalcitonin, RAGE, Resistin, Thrombomodulin, TNF alpha, TREM-1, Troponin I, uPAR, VCAM-1(CD106), VEGF-A
Species Reactivity
Human
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based Assay
Protocol Outline
Dry the array glass slide
Block glass slide surface
Incubate with samples
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Fluor Conjugated Streptavidin
Scan with an antibody array laser scanner
Analyze data
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction
The immune response is the body's response caused by its immune system being activated by antigens. The immune response can include immunity to pathogenic microorganisms and its products, allergies, graft rejections, as well as autoimmunity to self-antigens.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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