Influenzavirus A Recombinant H1N1 (A/Ohio/UR06-0091/2007) Hemagglutinin protein, His Tag (V2LY-0526-LY427)

Name: Influenzavirus A Recombinant H1N1 (A/Ohio/UR06-0091/2007) Hemagglutinin protein, His Tag
SKU: V2LY-0526-LY427
Rating: 5 (1 reviews)

See all HA antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Influenzavirus A

Tag

His Tag

Protein Construction

This product is Influenzavirus A Recombinant H1N1 (A/Ohio/UR06-0091/2007) Hemagglutinin protein, His Tag consist of Amino Acid: 1-528 and predicts a molecular mass of 59 kDa.

Molecule Mass

59 kDa

Sequence

Amino Acid: 1-528

Species

Influenzavirus A

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tirs, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Haemagglutinin

Function

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.

Biological Process

Clathrin-dependent endocytosis of virus by host cell Source: UniProtKB-UniRule
Fusion of virus membrane with host endosome membrane Source: UniProtKB-UniRule
Fusion of virus membrane with host plasma membrane Source: InterPro
Viral budding from plasma membrane Source: UniProtKB-UniRule
Virion attachment to host cell Source: UniProtKB-KW

Cellular Location

Apical cell membrane; Cell membrane; Host apical cell membrane; Membrane; Virion membrane. Targeted to the apical plasma membrane in epithelial polarized cells through a signal present in the transmembrane domain. Associated with glycosphingolipid- and cholesterol-enriched detergent-resistant lipid rafts.

Topology

Helical: 532-554

PTM

In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. One identified protease that may be involved in this process is secreted in lungs by Clara cells.
Palmitoylated.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Influenzavirus A Recombinant H1N1 (A/Ohio/UR06-0091/2007) Hemagglutinin protein, His Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Influenzavirus A Recombinant H10N3 (A/duck/Hong Kong/786/1979) Hemagglutinin protein, His Tag (CAT#: V2LY-0526-LY283)

Influenzavirus A Recombinant H10N3 (A/duck/Hong Kong/786/1979) Hemagglutinin protein, His Tag-1 (CAT#: V2LY-0526-LY284)

Influenzavirus A Recombinant H10N3 (A/duck/Hong Kong/786/1979) Hemagglutinin protein, His Tag-2 (CAT#: V2LY-0526-LY285)

Influenzavirus A Recombinant H10N3 (A/duck/Hunan/S11205/2012) Hemagglutinin protein, His Tag (CAT#: V2LY-0526-LY286)

Influenzavirus A Recombinant H10N3 (A/mallard/Minnesota/Sg-00194/2007) Hemagglutinin protein, His Tag (CAT#: V2LY-0526-LY287)

Influenzavirus A Recombinant H10N3 (A/mallard/Minnesota/Sg-00194/2007) Hemagglutinin protein, His Tag-1 (CAT#: V2LY-0526-LY288)

Influenzavirus A Recombinant H10N7 (A/blue-winged teal/Louisiana/Sg-00073/2007) Hemagglutinin protein, His Tag (CAT#: V2LY-0526-LY289)

Influenzavirus A Recombinant H10N7 (A/blue-winged teal/Louisiana/Sg-00073/2007) Hemagglutinin1 protein, His Tag (CAT#: V2LY-0526-LY290)

Influenzavirus A Recombinant H10N8 (A/duck/Guangdong/E1/2012) Hemagglutinin1 protein, His Tag (CAT#: V2LY-0526-LY291)

Influenzavirus A Recombinant H10N8 (A/Jiangxi-Donghu/346/2013) Hemagglutinin protein, His Tag (CAT#: V2LY-0526-LY293)

Related Products

Influenzavirus A Recombinant H3N2 (A/swine/Denmark/101501-1/2010) Hemagglutinin protein, His Tag (CAT#: V2LY-0526-LY706)

Influenzavirus A Recombinant H9N5 (A/shorebird/DE/261/2003) Hemagglutinin protein, His Tag (CAT#: V2LY-0526-LY1009)

Influenzavirus A Recombinant H3N2 (A/Aichi/2/1968) Hemagglutinin protein, ECD, His Tag (CAT#: V2LY-0526-LY575)

Influenzavirus A Recombinant H7N9 (A/Shanghai/1/2013) Hemagglutinin protein, His Tag (CAT#: V2LY-0526-LY976)

Influenzavirus A Recombinant H3N2 (A/Brisbane/10/2007) Hemagglutinin1 protein, His Tag (CAT#: V2LY-0526-LY593)

Influenza B Recombinant (B/Colorado/06/2017) Hemagglutinin protein, HA trimer , Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY1033)

Influenzavirus A Recombinant H3N2 (A/Hong Kong/1/1968) Hemagglutinin protein, ECD, His Tag (CAT#: V2LY-0526-LY637)

Influenzavirus A Recombinant H1N1 (A/Solomon Islands/3/2006) Hemagglutinin protein, His Tag (CAT#: V2LY-0526-LY443)

Influenzavirus A Recombinant H3N2 (A/Wisconsin/67/2005) Hemagglutinin protein, His Tag-1 (CAT#: V2LY-0526-LY736)

Influenzavirus A Recombinant H7N9 (A/Pigeon/Shanghai/S1069/2013) Hemagglutinin protein, ECD, His Tag (CAT#: V2LY-0526-LY970)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry