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Human Insulin Receptor Pathway Phosphorylation Antibody Array (Membrane, 9 Targets) (Y0551) (AbAr-0551-YC)


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Specifications

Target Array
Insulin Receptor Pathway Phosphorylation Array
Target
eIF-4E (pSer209), FOXO-3 (pSer413), IGF-1 R (pTyr1165), Insulin (pTyr1189), IRS-1 (pSer318), LKB1 (pSer428), SHC (pTyr427), SHIP1 (pTyr1020), SHP-2 (pSer542)
Species Reactivity
Human
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based Assay
Protocol Outline
Perform blocking of the array membrane surface.
Incubate with the sample.
Incubate using the detection antibody cocktail.
Proceed with incubation using HRP-anti-rabbit IgG antibodies.
Engage in incubation with detection buffers.
Acquire images with a chemiluminescent imaging system.
Conduct a comprehensive data analysis.
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction
The insulin transduction pathway is a biochemical pathway by which insulin increases the uptake of glucose into fat and muscle cells and reduces the synthesis of glucose in the liver and hence is involved in maintaining glucose homeostasis. This pathway is also influenced by fed versus fasting states, stress levels, and a variety of other hormones.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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