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Human Insulin Receptor Pathway Phosphorylation Antibody Array (Membrane, 9 Targets) (Y0551) (AbAr-0551-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The product provided herein is a Human Insulin Receptor Pathway Phosphorylation Antibody Array, which can be used for semi-quantitative detection of 9 human phosphorylated proteins. It is suitable for a range of liquid forms including Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates.
See all Insulin Receptor Pathway Phosphorylation antibodies

Specifications

Target Array
Insulin Receptor Pathway Phosphorylation Array
Target
eIF-4E (P-Ser209), FOXO-3 (P-Ser413), IGF-1 R (P-Tyr1165), Insulin (P-Tyr1189), IRS-1 (P-Ser318), LKB1 (P-Ser428), SHC (P-Tyr427), SHIP1 (P-Tyr1020), SHP-2 (P-Ser542)
Species Reactivity
Human
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based Assay
Protocol Outline
Block the array membrane surface
Incubate with Sample
Incubate with Detection Antibody Cocktail
Incubate with HRP Conjugated Anti-Rabbit IgG Antibodies
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Analyze data
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction
The insulin transduction pathway is a biochemical pathway by which insulin increases the uptake of glucose into fat and muscle cells and reduces the synthesis of glucose in the liver and hence is involved in maintaining glucose homeostasis. This pathway is also influenced by fed versus fasting states, stress levels, and a variety of other hormones.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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