Target Array
JAK/STAT Pathway Phosphorylation Array
Target
EGFR (P-Ser1070), JAK1 (P-Tyr1022), JAK2 (P-Tyr1007/1008), SHP-1 (P-Ser591), SHP2 (P-Tyr542), Src (P-Tyr419), STAT1 (P-Ser727), STAT2 (P-Tyr689), STAT3 (P-Tyr705), STAT5 (P-Tyr694), STAT6 (P-Tyr641), TYK2 (P-Tyr1054)
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates
Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Detection Assay
Sandwich-based Assay
Protocol Outline
Block the array membrane surface
Incubate with Sample
Incubate with Detection Antibody Cocktail
Incubate with HRP Conjugated Anti-Rabbit IgG Antibodies
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Analyze data
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.
Introduction
The JAK-STAT signalling pathway is a chain of interactions between proteins in a cell, and is involved in processes such as immunity, cell division, cell death and tumour formation. The pathway communicates information from chemical signals outside of a cell to the cell nucleus, resulting in the activation of genes through a process called transcription. There are three key parts of JAK-STAT signalling: Janus kinases (JAKs), signal transducer and activator of transcription proteins (STATs), and receptors (which bind the chemical signals). Disrupted JAK-STAT signalling may lead to a variety of diseases, such as skin conditions, cancers, and disorders affecting the immune system.
Datasheet 
