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Human JAK/STAT Pathway Phosphorylation Antibody Array (Membrane, 12 Targets) (Y0558) (AbAr-0558-YC)

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Specifications

Target Array
JAK/STAT Pathway Phosphorylation Array
Target
EGFR (pSer1070), JAK1 (pTyr1022), JAK2 (pTyr1007/1008), SHP-1 (pSer591), SHP2 (pTyr542), Src (pTyr419), STAT1 (pSer727), STAT2 (pTyr689), STAT3 (pTyr705), STAT5 (pTyr694), STAT6 (pTyr641), TYK2 (pTyr1054)
Species Reactivity
Human
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based Assay
Protocol Outline
Perform blocking of the array membrane surface.
Incubate with the sample.
Incubate using the detection antibody cocktail.
Proceed with incubation using HRP-anti-rabbit IgG antibodies.
Engage in incubation with detection buffers.
Acquire images with a chemiluminescent imaging system.
Conduct a comprehensive data analysis.
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction
The JAK-STAT signalling pathway is a chain of interactions between proteins in a cell, and is involved in processes such as immunity, cell division, cell death and tumour formation. The pathway communicates information from chemical signals outside of a cell to the cell nucleus, resulting in the activation of genes through a process called transcription. There are three key parts of JAK-STAT signalling: Janus kinases (JAKs), signal transducer and activator of transcription proteins (STATs), and receptors (which bind the chemical signals). Disrupted JAK-STAT signalling may lead to a variety of diseases, such as skin conditions, cancers, and disorders affecting the immune system.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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