Mouse Anti-1-methyladenosine (m1A) Recombinant Antibody (V2-9283) (CBMAB-1192-LY)
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RNAs were isolated from the total fraction (Total), mitochondrial fraction (Mito.), and non-mitochondrial fraction (Non-mito.) of mouse liver. (A) Each total RNA (0.6 μg) was separated in the 12% polyacrylamide gel and analyzed by SYBR staining and INB with anti-m1A antibody. The red arrowheads denote shorter tRNA bands, which were rich in the mitochondrial RNA. Arrows denote slight anti-m1A positive bands in the long exposure image. White arrowheads denote negative signals in 5.8S and 5S rRNA. (B) Each total RNA (1.2 μg) was separated on the 1% agarose gel and analyzed. The arrowheads denote positive signals in 28S and 18S rRNA. The arrow probably denotes the 28S rRNA-derived degraded transcript produced during the sample preparation. ...View More
Basic Information
Formulations & Storage [For reference only, actual COA shall prevail!]
Target
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Please try the standard protocols which include: protocols, troubleshooting and guide.
Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols
Custom Antibody Labeling
We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).
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