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Mouse Anti-AIFM2 Recombinant Antibody (V2-611175) (CBMAB-A0338-LY)

The product is antibody recognizes AMID. The antibody 3A11 immunoassay techniques such as: IP, ELISA.
See all AIFM2 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
V2-611175
Antibody Isotype
IgG1, κ
Application
IP, WB, IF, ELISA

Basic Information

Immunogen
Amino acids 1-300 mapping at the N-terminus of AMID of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Apoptosis Inducing Factor, Mitochondria Associated 2
Introduction
The protein encoded by this gene has significant homology to NADH oxidoreductases and the apoptosis-inducing factor PDCD8/AIF. Overexpression of this gene has been shown to induce apoptosis. The expression of this gene is found to be induced by tumor suppressor protein p53 in colon caner cells. [provided by RefSeq]
Entrez Gene ID
84883
UniProt ID
Q9BRQ8
Alternative Names
AMID; PRG3; RP11-367H5.2
Function
A NAD(P)H-dependent oxidoreductase involved in cellular oxidative stress response. At the plasma membrane, catalyzes reduction of coenzyme Q/ubiquinone-10 to ubiquinol-10, a lipophilic radical-trapping antioxidant that prevents lipid oxidative damage and consequently ferroptosis. Cooperates with GPX4 to suppress phospholipid peroxidation and ferroptosis. This anti-ferroptotic function is independent of cellular glutathione levels (PubMed:31634899, PubMed:31634900). May play a role in mitochondrial stress signaling. Upon oxidative stress, associates with the lipid peroxidation end product 4-hydroxy-2-nonenal (HNE) forming a lipid adduct devoid of oxidoreductase activity, which then translocates from mitochondria into the nucleus triggering DNA damage and cell death (PubMed:26689472). Capable of DNA binding in a non-sequence specific way (PubMed:15958387).
Biological Process
Apoptotic mitochondrial changes Source: MGI
Negative regulation of ferroptosis Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Regulation of apoptotic process Source: Reactome
Regulation of cellular response to oxidative stress Source: UniProtKB
Ubiquinone metabolic process Source: UniProtKB
Cellular Location
Cytoplasm; Cell membrane; Nucleus; Mitochondrion membrane; Lipid droplet
Topology
Helical: 7-27 aa
PTM
N-myristoylation at Gly-2 mediates the recruitment to lipid droplets and plasma membrane.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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