Mouse Anti-GNAT1 Recombinant Antibody (CBLG1-1402) (V2LY-0226-LY137)

Name: Mouse Anti-GNAT1 Recombinant Antibody (CBLG1-1402)
SKU: V2LY-0226-LY137
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBLG1-1402

Application

WB, ELISA, IF, IP

Immunogen

Amino acids 112-131 of mouse Gα t1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

G protein subunit alpha transducin 1

Entrez Gene ID

25686

UniProt ID

P10824

Function

Functions as signal transducer for the rod photoreceptor RHO. Required for normal RHO-mediated light perception by the retina (PubMed:22190596).

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs), such as the photoreceptor RHO. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Activated RHO promotes GDP release and GTP binding. Signaling is mediated via downstream effector proteins, such as cGMP-phosphodiesterase (By similarity).

Biological Process

Adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway Source: GO_Central
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
Cell population proliferation Source: Ensembl
Cellular response to electrical stimulus Source: Ensembl
Detection of chemical stimulus involved in sensory perception of bitter taste Source: Ensembl
Detection of light stimulus involved in visual perception Source: UniProtKB
Eye photoreceptor cell development Source: Ensembl
G protein-coupled adenosine receptor signaling pathway Source: GO_Central
Negative regulation of cyclic-nucleotide phosphodiesterase activity Source: UniProtKB
Phototransduction, visible light Source: UniProtKB
Positive regulation of cyclic-nucleotide phosphodiesterase activity Source: Ensembl
Regulation of rhodopsin mediated signaling pathway Source: Reactome
Response to light intensity Source: Ensembl
Response to light stimulus Source: UniProtKB
Retina development in camera-type eye Source: Ensembl
Rhodopsin mediated signaling pathway Source: Ensembl
Sensory perception of umami taste Source: Ensembl
Signal transduction Source: UniProtKB
Visual perception Source: UniProtKB

Cellular Location

Photoreceptor outer segment; Membrane; Photoreceptor inner segment. Localizes mainly in the outer segment in the dark-adapted state, whereas is translocated to the inner part of the photoreceptors in the light-adapted state. During dark-adapted conditions, in the presence of UNC119 mislocalizes from the outer segment to the inner part of rod photoreceptors which leads to decreased photoreceptor damage caused by light.

Involvement in disease

Night blindness, congenital stationary, autosomal dominant 3 (CSNBAD3):
A non-progressive retinal disorder characterized by impaired night vision, often associated with nystagmus and myopia.
Night blindness, congenital stationary, 1G (CSNB1G):
An autosomal recessive form of congenital stationary night blindness, a non-progressive retinal disorder characterized by impaired night vision or in dim light, with good vision only on bright days.

Schoonderwoerd, R. A., Buck, T. M., Andriessen, C. A., Wijnholds, J., Hattar, S., Meijer, J. H., & Deboer, T. (2022). Sleep Deprivation Does not Change the Flash Electroretinogram in Wild-type and Opn4−/− Gnat1−/− Mice. Journal of biological rhythms, 37(2), 216-221.

Hayashi, T., Hosono, K., Kurata, K., Katagiri, S., Mizobuchi, K., Ueno, S., ... & Hotta, Y. (2020). Coexistence of GNAT1 and ABCA4 variants associated with Nougaret-type congenital stationary night blindness and childhood-onset cone-rod dystrophy. Documenta Ophthalmologica, 140, 147-157.

Wang, J., Wang, W., Tang, Q., Lu, L., Luo, Z., Li, W., ... & Pu, J. (2020). Long Non-coding RNA lnc-GNAT1-1 Suppresses Liver Cancer Progression via Modulation of Epithelial–Mesenchymal Transition. Frontiers in Genetics, 11, 1029.

Zeitz, C., Méjécase, C., Stévenard, M., Michiels, C., Audo, I., & Marmor, M. F. (2018). A novel heterozygous missense mutation in GNAT1 leads to autosomal dominant Riggs type of congenital stationary night blindness. BioMed Research International, 2018.

Marmor, M. F., & Zeitz, C. (2018). Riggs-type dominant congenital stationary night blindness: ERG findings, a new GNAT1 mutation and a systemic association. Documenta Ophthalmologica, 137, 57-62.

Liu, L., Shuai, T., Li, B., Zhu, L., & Li, X. (2018). Long non‑coding RNA lnc‑GNAT1‑1 inhibits gastric cancer cell proliferation and invasion through the Wnt/β‑catenin pathway in Helicobacter pylori infection. Molecular Medicine Reports, 18(4), 4009-4015.

Zahid, S., Branham, K., Schlegel, D., Pennesi, M. E., Michaelides, M., Heckenlively, J., ... & Jayasundera, T. (2018). GNAT1. Retinal Dystrophy Gene Atlas, 101-101.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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