Rat Acan ELISA Kit (V2LY-0626-LY2042)

Name: Rat Acan ELISA Kit
SKU: V2LY-0626-LY2042
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0057 ng/mL

Detection Range

0.01-2 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Rat

Assay Type

Sandwich

Reactivity

Rat

Assay Time

1.5 h

Molecule Mass

221.1 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Aggrecan

Function

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

Biological Process

Cell adhesion
Central nervous system development
Extracellular matrix organization
Keratan sulfate biosynthetic process
Keratan sulfate catabolic process
Proteolysis
Skeletal system development

Cellular Location

Extracellular matrix

Involvement in disease

Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy.
A bone disease characterized by severe short stature, macrocephaly, severe midface hypoplasia, short neck, barrel chest and brachydactyly. The radiological findings comprise long bones with generalized irregular epiphyses with widened metaphyses, especially at the knees, platyspondyly, and multiple cervical-vertebral clefts.
An autosomal dominant disease characterized by short stature, advanced bone maturation, early-onset osteoarthritis, and mild dysmorphic features consisting of midface hypoplasia, brachydactyly, broad great toes, and lumbar lordosis. Other features include intervertebral disk disease and osteochondritis dissecans. Osteochondritis dissecans is defined as a separation of cartilage and subchondral bone from the surrounding tissue.

PTM

Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase.

König, K. C., Lahm, H., Dreßen, M., Doppler, S. A., Eichhorn, S., Beck, N., ... & Krane, M. (2021). Aggrecan: a new biomarker for acute type A aortic dissection. Scientific Reports, 11(1), 1-12.

Schmidt, S., Stapf, C., Schmutzler, S., Lachmann, I., Arendt, T., Holzer, M., ... & Morawski, M. (2020). Aggrecan modulates the expression and phosphorylation of tau in a novel bigenic TauP301L‐Acan mouse model. European Journal of Neuroscience.

Tashkandi, M. M., Alsaqer, S. F., Alhousami, T., Ali, F., Wu, Y. C., Shin, J., ... & Bais, M. V. (2020). LOXL2 promotes aggrecan and gender-specific anabolic differences to TMJ cartilage. Scientific reports, 10(1), 1-13.

de Andrade, D. G. A., Basso, R. M., Magro, A. J., Laufer-Amorim, R., Borges, A. S., & de Oliveira-Filho, J. P. (2020). Evaluation of a new variant in the aggrecan gene potentially associated with chondrodysplastic dwarfism in Miniature horses. Scientific reports, 10(1), 1-10.

Dupuis, L. E., Nelson, E. L., Hozik, B., Porto, S. C., Rogers-DeCotes, A., Fosang, A., & Kern, C. B. (2019). Adamts5−/− mice exhibit altered aggrecan proteolytic profiles that correlate with ascending aortic anomalies. Arteriosclerosis, thrombosis, and vascular biology, 39(10), 2067-2081.

Struck, A. K., Dierks, C., Braun, M., Hellige, M., Wagner, A., Oelmaier, B., ... & Distl, O. (2018). A recessive lethal chondrodysplasia in a miniature zebu family results from an insertion affecting the chondroitin sulfat domain of aggrecan. BMC genetics, 19(1), 1-9.

Zhang, B., Guo, W., Sun, C., Duan, H. Q., Yu, B. B., Mu, K., ... & Feng, S. Q. (2018). Dysregulated MiR-3150a-3p promotes lumbar intervertebral disc degeneration by targeting aggrecan. Cellular Physiology and Biochemistry, 45(6), 2506-2515.

Sentchordi‐Montané, L., Aza‐Carmona, M., Benito‐Sanz, S., Barreda‐Bonis, A. C., Sánchez‐Garre, C., Prieto‐Matos, P., ... & Heath, K. E. (2018). Heterozygous aggrecan variants are associated with short stature and brachydactyly: description of 16 probands and a review of the literature. Clinical endocrinology, 88(6), 820-829.

Rowlands, D., Lensjø, K. K., Dinh, T., Yang, S., Andrews, M. R., Hafting, T., ... & Dick, G. (2018). Aggrecan directs extracellular matrix-mediated neuronal plasticity. Journal of Neuroscience, 38(47), 10102-10113.

Gkourogianni, A., Andrew, M., Tyzinski, L., Crocker, M., Douglas, J., Dunbar, N., ... & Dauber, A. (2017). Clinical characterization of patients with autosomal dominant short stature due to aggrecan mutations. The Journal of Clinical Endocrinology & Metabolism, 102(2), 460-469.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human ACAN ELISA Kit (CAT#: V2LY-0626-LY4325)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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