Mouse Cd248 ELISA Kit (2) (V2LY-0626-LY888)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.025 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
81.8 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
CD248 Molecule
Function
May play a role in tumor angiogenesis.
Biological Process
Anatomical structure regression Source: Ensembl
Cell migration Source: GO_Central
Lymph node development Source: Ensembl
Positive regulation of cell population proliferation Source: Ensembl
Positive regulation of endothelial cell apoptotic process Source: Ensembl
Cellular Location
Membrane
Topology
Extracellular: 18-687
Helical: 688-708
Cytoplasmic: 709-757
PTM
O-glycosylated with sialylated oligosaccharides.
May be N-glycosylated.

Zhang, K., Xu, C., Liu, S., Jiang, Y., Zhao, X., Ma, S., ... & Qin, W. (2021). The Diagnostic and Immunotherapeutic Value of CD248 in Renal Cell Carcinoma. Frontiers in oncology, 11, 510.

Armitage, E. G., Barnes, A., Patrick, K., Bechar, J., Harrison, M. J., Lavery, G. G., ... & Naylor, A. J. (2021). Metabolic consequences for mice lacking Endosialin: LC–MS/MS-based metabolic phenotyping of serum from C56Bl/6J Control and CD248 knock‐out mice. Metabolomics, 17(2), 1-11.

Matsushima, S., Aoshima, Y., Akamatsu, T., Enomoto, Y., Meguro, S., Kosugi, I., ... & Iwashita, T. (2020). CD248 and integrin alpha-8 are candidate markers for differentiating lung fibroblast subtypes. BMC pulmonary medicine, 20(1), 1-15.

Park, G. B., Jeong, J. Y., & Kim, D. (2020). Modified TLR‐mediated downregulation of miR‐125b‐5p enhances CD248 (endosialin)‐induced metastasis and drug resistance in colorectal cancer cells. Molecular carcinogenesis, 59(2), 154-167.

Di Benedetto, P., Ruscitti, P., Liakouli, V., Del Galdo, F., Giacomelli, R., & Cipriani, P. (2019). Linking myofibroblast generation and microvascular alteration: the role of CD248 from pathogenesis to therapeutic target. Molecular medicine reports, 20(2), 1488-1498.

Teicher, B. A. (2019). CD248: A therapeutic target in cancer and fibrotic diseases. Oncotarget, 10(9), 993.

Petrus, P., Fernandez, T. L., Kwon, M. M., Huang, J. L., Lei, V., Safikhan, N. S., ... & Conway, E. M. (2019). Specific loss of adipocyte CD248 improves metabolic health via reduced white adipose tissue hypoxia, fibrosis and inflammation. EBioMedicine, 44, 489-501.

Di Benedetto, P., Liakouli, V., Ruscitti, P., Berardicurti, O., Carubbi, F., Panzera, N., ... & Giacomelli, R. (2018). Blocking CD248 molecules in perivascular stromal cells of patients with systemic sclerosis strongly inhibits their differentiation toward myofibroblasts and proliferation: a new potential target for antifibrotic therapy. Arthritis research & therapy, 20(1), 1-12.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Cd248 ELISA Kit (CAT#: V2LY-0626-LY2333)

Human CD248 ELISA Kit (CAT#: V2LY-0626-LY4542)

CD248 Matched Antibody Pair (211) (CAT#: APMAB-211LY)

CD248 Matched Antibody Pair (212) (CAT#: APMAB-212LY)

Antibody Pairs

CD248 Matched Antibody Pair (211) (CAT#: APMAB-211LY)

CD248 Matched Antibody Pair (212) (CAT#: APMAB-212LY)

Related Products

Mouse Anti-CD248 Recombinant Antibody (CBFYC-1178) (CAT#: CBMAB-C1236-FY)

Human Anti-CD248 Recombinant Antibody (CBFYC-1179) (CAT#: CBMAB-C1237-FY)

Mouse Anti-CD248 Recombinant Antibody (C6683) (CAT#: CBMAB-C6683-LY)

Mouse Cd248 ELISA Kit (CAT#: V2LY-0626-LY2333)

Mouse Anti-CD248 Recombinant Antibody (CBFYE-0327) (CAT#: CBMAB-E0613-FY)

Mouse Anti-CD248 Recombinant Antibody (CBT2897) (CAT#: V2LY-0625-LY1138)

CD248 Matched Antibody Pair (211) (CAT#: APMAB-211LY)

Rat Anti-CD248 Recombinant Antibody (CBXC-1509) (CAT#: CBMAB-C3606-CQ)

Human CD248 ELISA Kit (CAT#: V2LY-0626-LY4542)

CD248 Matched Antibody Pair (212) (CAT#: APMAB-212LY)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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