Mouse Enpp2 ELISA Kit (V2LY-0626-LY2328)

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Tested Data
Datasheet
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Basic Information

Sensitivity
9.52 ng/mL
Detection Range
20-4500 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
98.9 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Ectonucleotide Pyrophosphatase/Phosphodiesterase 2
Research Area
Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:15769751, PubMed:26371182, PubMed:27754931, PubMed:14500380, PubMed:12354767,).

Major substrate is lysophosphatidylcholine (PubMed:12176993, PubMed:27754931, PubMed:14500380).

Also can act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility (PubMed:14500380).

Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP (PubMed:15769751, PubMed:12176993).

Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation (PubMed:11559573).

Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein (PubMed:1733949).

May have a role in induction of parturition (PubMed:12176993).

Possible involvement in cell proliferation and adipose tissue development (Probable). Tumor cell motility-stimulating factor (PubMed:1733949, PubMed:11559573).

Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids (PubMed:21393252).

Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) (PubMed:21393252).
Biological Process
Cell motility Source: UniProtKB
Chemotaxis Source: UniProtKB
Immune response Source: InterPro
Phosphatidylcholine catabolic process Source: UniProtKB
Phospholipid catabolic process Source: UniProtKB
Positive regulation of epithelial cell migration Source: UniProtKB
Positive regulation of lamellipodium morphogenesis Source: UniProtKB
Positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
Regulation of angiogenesis Source: InterPro
Regulation of cell migration Source: UniProtKB
Sphingolipid catabolic process Source: UniProtKB
Cellular Location
Secreted
PTM
N-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity.
The interdomain disulfide bond between Cys-414 and Cys-806 is essential for catalytic activity.

Karshovska, E., Mohibullah, R., Zhu, M., Zahedi, F., Thomas, D., Magkrioti, C., ... & Schober, A. (2022). Endothelial ENPP2 (Ectonucleotide Pyrophosphatase/Phosphodiesterase 2) Increases Atherosclerosis in Female and Male Mice. Arteriosclerosis, Thrombosis, and Vascular Biology, 42(8), 1023-1036.

Borza, R., Salgado-Polo, F., Moolenaar, W. H., & Perrakis, A. (2022). Structure and function of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family: Tidying up diversity. Journal of Biological Chemistry, 298(2).

Li, Y., Zhang, L., Xu, T., Zhao, X., Jiang, X., Xiao, F., ... & Wang, L. (2022). Aberrant ENPP2 expression promotes tumor progression in multiple myeloma. Leukemia & Lymphoma, 63(4), 963-974.

Mamoor, S. (2021). Differential expression of ectonucleotide pyrophosphatase/phosphodiesterase 2 in cancers of the breast.

He, L., Yang, Y., Chen, J., Zou, P., & Li, J. (2021). Transcriptional activation of ENPP2 by FoxO4 protects cardiomyocytes from doxorubicin‑induced toxicity. Molecular Medicine Reports, 24(3), 1-11.

Deng, W., Chen, F., Zhou, Z., Huang, Y., Lin, J., Zhang, F., ... & Xu, L. (2021). Hepatitis B virus promotes hepatocellular carcinoma progression synergistically with hepatic stellate cells via facilitating the expression and secretion of ENPP2. Frontiers in molecular biosciences, 8.

Sah, J. P., Hao, N. T. T., Han, X., Tran, T. T. T., McCarthy, S., Oh, Y., & Yoon, J. K. (2020). Ectonucleotide pyrophosphatase 2 (ENPP2) plays a crucial role in myogenic differentiation through the regulation by WNT/β-catenin signaling. The international journal of biochemistry & cell biology, 118, 105661.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant ENPP2, Active protein, GST Tag (CAT#: V2LY-0526-LY2217)

Human Recombinant ENPP2 protein, His Tag (CAT#: V2LY-0526-LY2218)

Human Recombinant ENPP2 protein, His Tag-1 (CAT#: V2LY-0526-LY2219)

Cynomolgus Recombinant ENPP2 protein, His Tag (CAT#: V2LY-0526-LY1379)

Mouse Recombinant ENPP2 protein, His Tag (CAT#: V2LY-0526-LY7831)

Related Products

Mouse Anti-ENPP2 Monoclonal Antibody (1F8) (CAT#: CBMAB-1155-YC)

Mouse Anti-ENPP2 Recombinant Antibody (CAP698) (CAT#: CBMAB-AP2068LY)

Human Recombinant ENPP2, Active protein, GST Tag (CAT#: V2LY-0526-LY2217)

Mouse Recombinant ENPP2 protein, His Tag (CAT#: V2LY-0526-LY7831)

Human Recombinant ENPP2 protein, His Tag-1 (CAT#: V2LY-0526-LY2219)

Rabbit Anti-ENPP2 Monoclonal Antibody (5H3) (CAT#: CBMAB-1154-YC)

Human Recombinant ENPP2 protein, His Tag (CAT#: V2LY-0526-LY2218)

Cynomolgus Recombinant ENPP2 protein, His Tag (CAT#: V2LY-0526-LY1379)

Mouse Anti-ENPP2 Recombinant Antibody (CBFYE-0891) (CAT#: CBMAB-E1356-FY)

Isotype control

For research use only. Not intended for any clinical use.

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