Pig ADRB2 ELISA Kit (V2LY-0626-LY932)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.0094 ng/mL
Detection Range
0.02-4.5 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Pig
Assay Type
Sandwich
Reactivity
Pig
Assay Time
1.5 h
Molecule Mass
46.8 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Adrenoceptor Beta 2
Function
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
Cellular Location
Early endosome; Cell membrane; Golgi apparatus. Colocalizes with VHL at the cell membrane. Activated receptors are internalized into endosomes prior to their degradation in lysosomes. Activated receptors are also detected within the Golgi apparatus.
Topology
Extracellular: 1-34 aa
Helical: 35-58 aa
Cytoplasmic: 59-71 aa
Helical: 72-95 aa
Extracellular: 96-106 aa
Helical: 107-129 aa
Cytoplasmic: 130-150 aa
Helical: 151-174 aa
Extracellular: 175-196 aa
Helical: 197-220 aa
Cytoplasmic: 221-274 aa
Helical: 275-298 aa
Extracellular: 299-305 aa
Helical: 306-329 aa
Cytoplasmic: 330-413 aa
PTM
Palmitoylated. Mainly palmitoylated at Cys-341. Palmitoylation may reduce accessibility of phosphorylation sites by anchoring the receptor to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation. Also undergoes transient, ligand-induced palmitoylation at Cys-265 probably by ZDHHC9, ZDHHC14 and ZDHHC18 within the Golgi. Palmitoylation at Cys-265 requires phosphorylation by PKA and receptor internalization and stabilizes the receptor. Could be depalmitoylated by LYPLA1 at the plasma membrane.
Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK.
Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
Polyubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation. Ubiquitination by the VHL-E3 ligase complex is oxygen-dependent.
Hydroxylation by EGLN3 oCcurs only under normoxia and increases the interaction with VHL and the subsequent ubiquitination and degradation of ADRB2.

Hikino, K., Kobayashi, S., Ota, E., Mushiroda, T., Urayama, K. Y., & Kobayashi, T. (2021). A meta‐analysis of the influence of ADRB2 genetic polymorphisms on albuterol (salbutamol) therapy in patients with asthma. British Journal of Clinical Pharmacology, 87(4), 1708-1716.

Wang, Y., & Jiang, S. (2021). The role of ADRB2 gene polymorphisms in malignancies. Molecular Biology Reports, 1-9.

Lehrer, S., & Rheinstein, P. H. (2020). The ADRB1 (Adrenoceptor Beta 1) and ADRB2 Genes Significantly Co-express with Commonly Mutated Genes in Prostate Cancer. Discovery medicine, 30(161), 163.

Karimi, L., Lahousse, L., Ghanbari, M., Terzikhan, N., Uitterlinden, A. G., van der Lei, J., ... & Verhamme, K. (2019). β2-Adrenergic Receptor (ADRB2) Gene Polymorphisms and Risk of COPD Exacerbations: The Rotterdam Study. Journal of clinical medicine, 8(11), 1835.

Toraih, E. A., Hussein, M. H., Ibrahim, A., AbdAllah, N. B., Mohammad, E., Kishk, A. M., & Fawzy, M. S. (2019). Beta2-adrenergic receptor variants in children and adolescents with bronchial asthma. Front Biosci (Elite Ed), 11, 61-78.

Wang, L., Lv, Q., Song, X., Jiang, K., & Zhang, J. (2019). ADRB2 suppresses IL-13-induced allergic rhinitis inflammatory cytokine regulated by miR-15a-5p. Human cell, 32(3), 306-315.

Zhang, X., Zhang, Y., He, Z., Yin, K., Li, B., Zhang, L., & Xu, Z. (2019). Chronic stress promotes gastric cancer progression and metastasis: An essential role for ADRB2. Cell death & disease, 10(11), 1-15.

Ágg, B., Baranyai, T., Makkos, A., Vető, B., Faragó, N., Zvara, Á., ... & Ferdinandy, P. (2018). MicroRNA interactome analysis predicts post-transcriptional regulation of ADRB2 and PPP3R1 in the hypercholesterolemic myocardium. Scientific reports, 8(1), 1-11.

Wang, X., Li, Q., Liu, R., He, J., Wu, D., Wang, Y., & Zhang, J. (2018). ADRB2 Arg16Gly polymorphism and pulmonary function response of inhaled corticosteroids plus long-acting beta agonists for Asthma treatment: A systematic review and meta-analysis. Canadian respiratory journal, 2018.

Nielsen, A. O., Jensen, C. S., Arredouani, M. S., Dahl, R., & Dahl, M. (2017). Variants of the ADRB2 gene in COPD: systematic review and meta-analyses of disease risk and treatment response. COPD: Journal of Chronic Obstructive Pulmonary Disease, 14(4), 451-460.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rat Adrb2 ELISA Kit (CAT#: V2LY-0626-LY1540)

Mouse Adrb2 ELISA Kit (CAT#: V2LY-0626-LY2075)

Human ADRB2 ELISA Kit (CAT#: V2LY-0626-LY5508)

Related Products

Human ADRB2 ELISA Kit (CAT#: V2LY-0626-LY5508)

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Rat Adrb2 ELISA Kit (CAT#: V2LY-0626-LY1540)

Mouse Anti-ADRB2 Recombinant Antibody (V2-606602) (CAT#: CBMAB-X0259-FY)

Mouse Anti-ADRB2 Recombinant Antibody (CBT3524) (CAT#: V2LY-0625-LY570)

Mouse Anti-ADRB2 Recombinant Antibody (CBT3335) (CAT#: V2LY-0625-LY569)

Mouse Adrb2 ELISA Kit (CAT#: V2LY-0626-LY2075)

Mouse Anti-ADRB2 Recombinant Antibody (V2-180025) (CAT#: CBMAB-A1419-YC)

Rabbit Anti-ADRB2 Recombinant Antibody (
4C11) (CAT#: V2LY-0725-LY1163)

Isotype control

For research use only. Not intended for any clinical use.

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