Mouse Itsn1 ELISA Kit (V2LY-0626-LY471)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.57 ng/mL
Detection Range
1-380 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
194.3 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
intersectin 1 (SH3 domain protein)
Function
Adapter protein that provides a link between the endocytic membrane traffic and the actin assembly machinery (PubMed:11584276, PubMed:29887380).
Acts as guanine nucleotide exchange factor (GEF) for CDC42, and thereby stimulates actin nucleation mediated by WASL and the ARP2/3 complex (PubMed:11584276).
Plays a role in the assembly and maturation of clathrin-coated vesicles (By similarity).
Recruits FCHSD2 to clathrin-coated pits (PubMed:29887380).
Involved in endocytosis of activated EGFR, and probably also other growth factor receptors (By similarity).
Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2 (PubMed:22648170).
Promotes ubiquitination and subsequent degradation of EGFR, and thereby contributes to the down-regulation of EGFR-dependent signaling pathways. In chromaffin cells, required for normal exocytosis of catecholamines. Required for rapid replenishment of release-ready synaptic vesicles at presynaptic active zones (By similarity).
Inhibits ARHGAP31 activity toward RAC1 (PubMed:11744688).
Isoform 1
Plays a role in synaptic vesicle endocytosis in brain neurons.
Biological Process
Cellular protein localizationManual Assertion Based On ExperimentIMP:UniProtKB
Clathrin-dependent synaptic vesicle endocytosisManual Assertion Based On ExperimentIBA:GO_Central
EndocytosisManual Assertion Based On ExperimentIBA:GO_Central
Endosomal transportManual Assertion Based On ExperimentIBA:GO_Central
ExocytosisIEA:UniProtKB-KW
Intracellular signal transductionIEA:InterPro
Protein transportIEA:UniProtKB-KW
Regulation of small GTPase mediated signal transductionTAS:Reactome
Cellular Location
Endomembrane system; Cell junction, synapse, synaptosome; Cell projection, lamellipodium; Cell membrane; Membrane, clathrin-coated pit; Recycling endosome; Endosome; Cytoplasmic vesicle. Colocalizes with SGIP1 at the plasma membrane in structures corresponding most probably to clathrin-coated pits (PubMed:20946875).
Colocalizes with RAB13 on cytoplasmic vesicles that are most likely recycling endosomes (PubMed:29030480).
Isoform 2: Cytoplasm; Endomembrane system; Nucleus envelope. Shuttles between the cytoplasm and nucleus in an XPO1/CRM1-dependent manner.
Isoform 5: Endomembrane system

Takizawa, C., Minematsu, T., Nakagami, G., Kitamura, A., Koudounas, S., Kunimitsu, M., & Sanada, H. (2023). Expression levels of NPPB, ITGB6, CPNE4, EML5, and ITSN1 in fresh exudates swabbed from critically colonised and infected full‐thickness wounds in rats. International Wound Journal, 20(4), 1088-1097.

Wang, G., Zhou, Y., Zhong, T., Song, A., & Xue, Q. (2022). Longitudinal variation of circulating Inc‐ITSN1‐2: A novel biomarker reflecting disease severity, inflammation, recurrence, and death risk in acute ischemic stroke patients. Journal of Clinical Laboratory Analysis, 36(6), e24468.

Bruel, A. L., Vitobello, A., Thiffault, I., Manwaring, L., Willing, M., Agrawal, P. B., ... & Faivre, L. (2022). ITSN1: a novel candidate gene involved in autosomal dominant neurodevelopmental disorder spectrum. European Journal of Human Genetics, 30(1), 111-116.

Mishra, R., Sengül, G. F., Candiello, E., & Schu, P. (2021). Synaptic AP2 CCV life cycle regulation by the Eps15, ITSN1, Sgip1/AP2, synaptojanin1 interactome. Scientific Reports, 11(1), 8007.

Zong, X., Xiao, X., Kai, L., Cheng, Y., Fu, J., Xu, W., ... & Jin, M. (2021). Atractylodis macrocephalae polysaccharides protect against DSS-induced intestinal injury through a novel lncRNA ITSN1-OT1. International Journal of Biological Macromolecules, 167, 76-84.

Pankivskyi, S., Pastré, D., Steiner, E., Joshi, V., Rynditch, A., & Hamon, L. (2021). ITSN1 regulates SAM68 solubility through SH3 domain interactions with SAM68 proline-rich motifs. Cellular and Molecular Life Sciences, 78, 1745-1763.

Nie, J., & Zhao, Q. (2020). Lnc-ITSN1-2, derived from RNA sequencing, correlates with increased disease risk, activity and promotes CD4+ T cell activation, proliferation and Th1/Th17 cell differentiation by serving as a ceRNA for IL-23R via sponging miR-125a in inflammatory bowel disease. Frontiers in immunology, 11, 852.

Zeng, Q., Wu, J., & Yang, S. (2019). Circulating lncRNA ITSN1‐2 is upregulated, and its high expression correlates with increased disease severity, elevated inflammation, and poor survival in sepsis patients. Journal of Clinical Laboratory Analysis, 33(4), e22836.

Yue, T., Fan, X., Zhang, Z., Liu, Z., Guo, M., Bai, F., ... & Xiao, L. (2019). Downregulation of lncRNA ITSN1-2 correlates with decreased disease risk and activity of rheumatoid arthritis (RA), and reduces RA fibroblast-like synoviocytes proliferation and inflammation via inhibiting NOD2/RIP2 signaling pathway. American journal of translational research, 11(8), 4650.

Gryaznova, T., Gubar, O., Burdyniuk, M., Kropyvko, S., & Rynditch, A. (2018). WIP/ITSN1 complex is involved in cellular vesicle trafficking and formation of filopodia-like protrusions. Gene, 674, 49-56.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
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