Mouse Kng1 ELISA Kit (V2LY-0626-LY1663)

Name: Mouse Kng1 ELISA Kit
SKU: V2LY-0626-LY1663
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

1.62 ng/mL

Detection Range

3-900 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Mouse

Assay Type

Sandwich

Reactivity

Mouse

Assay Time

1.5 h

Molecule Mass

73.1 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Kininogen 1

Function

(1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.

Biological Process

Antimicrobial humoral immune response mediated by antimicrobial peptideIDA:UniProtKB
Blood coagulationIEA:UniProtKB-KW
Inflammatory responseIEA:UniProtKB-KW
Killing of cells of other organismIDA:UniProtKB
Negative regulation of blood coagulationManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of cell adhesionManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of endopeptidase activityManual Assertion Based On ExperimentIBA:GO_Central
Negative regulation of proteolysisManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of apoptotic process1 PublicationNAS:UniProtKB
Positive regulation of cytosolic calcium ion concentrationManual Assertion Based On ExperimentIDA:UniProtKB
VasodilationIEA:UniProtKB-KW

Cellular Location

Secreted, extracellular space

Involvement in disease

High molecular weight kininogen deficiency (HMWK deficiency):
Autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.

PTM

Bradykinin is released from kininogen by plasma kallikrein.
Hydroxylation of Pro-383 ^oCcurs prior to the release of bradykinin.
Phosphorylated by FAM20C in the extracellular medium.
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

Adenaeuer, A., Barco, S., Trinchero, A., Krutmann, S., Nazir, H. F., Ambaglio, C., ... & Rossmann, H. (2023). Severe high-molecular-weight kininogen deficiency: clinical characteristics, deficiency–causing KNG1 variants, and estimated prevalence. Journal of thrombosis and haemostasis, 21(2), 237-254.

Cheng, X., Liu, D., Song, H., Tian, X., Yan, C., & Han, Y. (2021). Overexpression of Kininogen-1 aggravates oxidative stress and mitochondrial dysfunction in DOX-induced cardiotoxicity. Biochemical and biophysical research communications, 550, 142-150.

Vijay, A., Kassab, M. B., Shim, Y. J., Swaidani, S., Mauskapf, A., McCrae, K. R., & Jaffer, F. A. (2021). Protective Effects of Kininogen-1 Gene Deficiency in Mouse Models of Venous Thrombosis. Blood, 138, 289.

Adenaeuer, A., Barco, S., Trinchero, A., Nazir, H., Krutmann, S., Ambaglio, C., ... & Lämmle, B. (2021). Severe High Molecular Weight Kininogen (HK) Deficiency: Clinical Characteristics, Deficiency-Causing KNG1 Variants in Reported and New Cases, and Estimated Prevalence. Blood, 138(Supplement 1), 3200-3200.

Jeong, D., Goo, J. Y., Kim, H. K., Chong, S. Y., & Kang, M. S. (2020). The first korean case of high-molecular-weight kininogen deficiency, with a novel variant, c. 488delG, in the KNG1 gene. Annals of laboratory medicine, 40(3), 264.

Wang, W., Wang, S., & Zhang, M. (2020). Evaluation of kininogen 1, osteopontin and α‑1‑antitrypsin in plasma, bronchoalveolar lavage fluid and urine for lung squamous cell carcinoma diagnosis. Oncology letters, 19(4), 2785-2792.

Yu, J., Huang, Y., Lin, C., Li, X., Fang, X., Zhong, C., ... & Zheng, S. (2018). Identification of kininogen 1 as a serum protein marker of colorectal adenoma in patients with a family history of colorectal cancer. Journal of Cancer, 9(3), 540.

Xu, J., Fang, J., Cheng, Z., Fan, L., Hu, W., Zhou, F., & Shen, H. (2018). Overexpression of the Kininogen-1 inhibits proliferation and induces apoptosis of glioma cells. Journal of Experimental & Clinical Cancer Research, 37, 1-15.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rat Kng1 ELISA Kit (CAT#: V2LY-0626-LY1890)

Human KNG1 ELISA Kit (CAT#: V2LY-0626-LY3766)

Human Recombinant KNG1 protein, His Tag (CAT#: V2LY-0526-LY5164)

Mouse Recombinant KNG1 protein, His Tag (CAT#: V2LY-0526-LY8590)

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Human Recombinant KNG1 protein, His Tag (CAT#: V2LY-0526-LY5164)

Isotype control

For research use only. Not intended for any clinical use.

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