Mouse Recombinant BST2 protein, hFc Tag (V2LY-0526-LY7885)

Name: Mouse Recombinant BST2 protein, hFc Tag
SKU: V2LY-0526-LY7885
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

hFc Tag

Protein Construction

This product is Mouse Recombinant BST2 protein, hFc Tag consist of Amino Acid: 52-151 and predicts a molecular mass of 40 kDa.

Molecule Mass

40 kDa

Sequence

Amino Acid: 52-151

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tirs, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Bone Marrow Stromal Cell Antigen 2

Alternative Names

Bone Marrow Stromal Cell Antigen 2; HM1.24 Antigen; TETHERIN; BST-2; Bone Marrow Stromal Antigen 2; CD317 Antigen; NPC-A-7; CD317;

Function

IFN-induced antiviral host restriction factor which efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. Acts as a direct physical tether, holding virions to the cell membrane and linking virions to each other. The tethered virions can be internalized by endocytosis and subsequently degraded or they can remain on the cell surface. In either case, their spread as cell-free virions is restricted (PubMed:22520941, PubMed:21529378, PubMed:20940320, PubMed:20419159, PubMed:20399176, PubMed:19879838, PubMed:19036818, PubMed:18342597, PubMed:18200009).
Its target viruses belong to diverse families, including retroviridae: human immunodeficiency virus type 1 (HIV-1), human immunodeficiency virus type 2 (HIV-2), simian immunodeficiency viruses (SIVs), equine infectious anemia virus (EIAV), feline immunodeficiency virus (FIV), prototype foamy virus (PFV), Mason-Pfizer monkey virus (MPMV), human T-cell leukemia virus type 1 (HTLV-1), Rous sarcoma virus (RSV) and murine leukemia virus (MLV), flavivirideae: hepatitis C virus (HCV), filoviridae: ebola virus (EBOV) and marburg virus (MARV), arenaviridae: lassa virus (LASV) and machupo virus (MACV), herpesviridae: kaposis sarcoma-associated herpesvirus (KSHV), rhabdoviridae: vesicular stomatitis virus (VSV), orthomyxoviridae: influenza A virus, paramyxoviridae: nipah virus, and coronaviridae: SARS-CoV (PubMed:22520941, PubMed:21621240, PubMed:21529378, PubMed:20943977, PubMed:20686043, PubMed:20419159, PubMed:20399176, PubMed:19879838, PubMed:19179289, PubMed:18342597, PubMed:18200009, PubMed:26378163, PubMed:31199522).
Can inhibit cell surface proteolytic activity of MMP14 causing decreased activation of MMP15 which results in inhibition of cell growth and migration (PubMed:22065321).
Can stimulate signaling by LILRA4/ILT7 and consequently provide negative feedback to the production of IFN by plasmacytoid dendritic cells in response to viral infection (PubMed:19564354, PubMed:26172439).
Plays a role in the organization of the subapical actin cytoskeleton in polarized epithelial cells. Isoform 1 and isoform 2 are both effective viral restriction factors but have differing antiviral and signaling activities (PubMed:23028328, PubMed:26172439).
Isoform 2 is resistant to HIV-1 Vpu-mediated degradation and restricts HIV-1 viral budding in the presence of Vpu (PubMed:23028328, PubMed:26172439).
Isoform 1 acts as an activator of NF-kappa-B and this activity is inhibited by isoform 2 (PubMed:23028328).

Biological Process

B cell activation Source: UniProtKB-KW
Defense response to virus Source: UniProtKB
Innate immune response Source: UniProtKB
Negative regulation of cell growth Source: UniProtKB
Negative regulation of cell migration Source: UniProtKB
Negative regulation of intracellular transport of viral material Source: UniProtKB
Negative regulation of plasmacytoid dendritic cell cytokine production Source: UniProtKB
Negative regulation of viral genome replication Source: UniProtKB
Neutrophil degranulation Source: Reactome
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Positive regulation of leukocyte proliferation Source: GO_Central
Regulation of actin cytoskeleton organization Source: UniProtKB
Response to interferon-alpha Source: UniProtKB
Response to interferon-beta Source: UniProtKB
Response to interferon-gamma Source: UniProtKB
Response to virus Source: UniProtKB
Type I interferon signaling pathway Source: Reactome

Cellular Location

Trans-Golgi network; Cytoplasm; Cell membrane; Apical cell membrane; Membrane raft. Shuttles between the cell membrane, where it is present predominantly in membrane/lipid rafts, and the trans-Golgi network. Forms a complex with MMP14 and localizes to the cytoplasm.
Trans-Golgi network; Late endosome. (Microbial infection) HIV-1 VPU and HIV-2 ENV can target it to the trans-Golgi network thus sequestering it away from virus assembly sites on the cell membrane. Targeted to late endosomes upon KSHV infection and subsequent ubiquitination.

Topology

Cytoplasmic: 1-20 aa
Helical: 21-48 aa
Extracellular: 49-161 aa

PTM

Monoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation.1 Publication
The GPI anchor is essential for its antiviral activity.

Bian, S., Zhao, Y., Li, F., Lu, S., He, Z., Wang, S., ... & Wang, J. (2021). Total ginsenosides induce autophagic cell death in cervical cancer cells accompanied by downregulation of bone marrow stromal antigen‑2. Experimental and Therapeutic Medicine, 22(1), 1-9.

Mlimi, H., Naidoo, K. K., Mabuka, J., Ndung’u, T., & Madlala, P. (2021). Bone marrow stromal antigen 2 (BST-2) genetic variants influence expression levels and disease outcome in HIV-1 chronically infected patients.

Kulkarni, R., Jiang, S., Birrane, G., & Prasad, A. (2020). Lymphocyte‐specific protein 1 (LSP1) regulates bone marrow stromal cell antigen 2 (BST‐2)‐mediated intracellular trafficking of HIV‐1 in dendritic cells. FEBS letters, 594(12), 1947-1959.

Dias, B. D. C., Paximadis, M., Martinson, N., Chaisson, R. E., Ebrahim, O., & Tiemessen, C. T. (2020). The impact of bone marrow stromal antigen-2 (BST2) gene variants on HIV-1 control in black South African individuals. Infection, Genetics and Evolution, 80, 104216.

Shi, H., Luo, K., & Huang, W. (2020). Bone Marrow Stromal Antigen 2 is a Potential Unfavorable Prognostic Factor for High-Grade Glioma. OncoTargets and therapy, 13, 8723.

Florez, M. A., Matatall, K. A., Jeong, Y., Ortinau, L., Shafer, P., Lynch, A., ... & King, K. Y. (2020). Bone Marrow Stromal Antigen 2 Is Critical for IFNy-Dependent Hematopoietic Stem Cell Activation. Blood, 136, 2-3.

Liu, W., Cao, Y., Guan, Y., & Zheng, C. (2018). BST2 promotes cell proliferation, migration and induces NF-κB activation in gastric cancer. Biotechnology letters, 40(7), 1015-1027.

Chen, C., Shi, H., Wang, B., Cao, N., Yu, C., & Zheng, L. (2018). Aberrant expression of the innate restriction factor bone marrow stromal antigen-2 in primary Sjögren's syndrome. Journal of Cranio-Maxillofacial Surgery, 46(11), 1899-1904.

Wang, J., Bian, S., Liu, M., Zhang, X., Wang, S., Bai, X., ... & Zhao, Y. (2018). Cloning, identification, and functional analysis of bone marrow stromal cell antigen-2 from sika deer (Cervus nippon). Gene, 661, 133-138.

Haga, Y., Kanda, T., Nakamoto, S., Nakamura, M., Sasaki, R., Wu, S., & Yokosuka, O. (2017). Interferon induces interleukin 8 and bone marrow stromal cell antigen 2 expression, inhibiting the production of hepatitis B virus surface antigen from human hepatocytes. Biochemical and biophysical research communications, 486(3), 858-863.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant BST2 protein, hFc Tag (CAT#: V2LY-0526-LY2417)

Human Recombinant BST2 protein, His Tag (CAT#: V2LY-0526-LY2418)

Mouse Recombinant BST2 protein, ECD, His Tag (CAT#: V2LY-0526-LY7884)

BST2 Matched Antibody Pair (145) (CAT#: APMAB-145LY)

BST2 Matched Antibody Pair (146) (CAT#: APMAB-146LY)

BST2 Matched Antibody Pair (147) (CAT#: APMAB-147LY)

BST2 Matched Antibody Pair (148) (CAT#: APMAB-148LY)

BST2 Matched Antibody Pair (149) (CAT#: APMAB-149LY)

Antibody Pairs

BST2 Matched Antibody Pair (145) (CAT#: APMAB-145LY)

BST2 Matched Antibody Pair (146) (CAT#: APMAB-146LY)

BST2 Matched Antibody Pair (147) (CAT#: APMAB-147LY)

BST2 Matched Antibody Pair (148) (CAT#: APMAB-148LY)

BST2 Matched Antibody Pair (149) (CAT#: APMAB-149LY)

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Human Recombinant BST2 protein, hFc Tag (CAT#: V2LY-0526-LY2417)

Human Recombinant BST2 protein, His Tag (CAT#: V2LY-0526-LY2418)

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For research use only. Not intended for any clinical use.

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