Mouse Recombinant GRN protein, His Tag (V2LY-0526-LY8798)

Name: Mouse Recombinant GRN protein, His Tag
SKU: V2LY-0526-LY8798
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant GRN protein, His Tag consist of Amino Acid: 1-589 and predicts a molecular mass of 63 kDa.

Molecule Mass

63 kDa

Sequence

Amino Acid: 1-589

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Granulin Precursor

Function

Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation (PubMed:28541286, PubMed:28073925, PubMed:18378771, PubMed:28453791, PubMed:12526812).

Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes (PubMed:28453791, PubMed:28541286).

Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB (PubMed:28073925).

In addition, functions as wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures (By similarity).

Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases (PubMed:12526812).

Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity (PubMed:18378771).

Granulin-4:
Promotes proliferation of the epithelial cell line A431 in culture.

Granulin-3:
Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8.

Granulin-7:
Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity.

Biological Process

Astrocyte activation involved in immune response Source: UniProtKB
Lysosomal lumen acidification Source: UniProtKB
Lysosomal transport Source: UniProtKB
Lysosome organization Source: UniProtKB
Microglial cell activation involved in immune response Source: UniProtKB
Negative regulation of microglial cell activation Source: UniProtKB
Negative regulation of neuron apoptotic process Source: UniProtKB
Negative regulation of neutrophil activation Source: UniProtKB
Negative regulation of respiratory burst involved in inflammatory response Source: UniProtKB
Positive regulation of angiogenesis Source: UniProtKB
Positive regulation of aspartic-type peptidase activity Source: UniProtKB
Positive regulation of axon regeneration Source: UniProtKB
Positive regulation of cell migration Source: ARUK-UCL
Positive regulation of defense response to bacterium Source: UniProtKB
Positive regulation of endothelial cell migration Source: UniProtKB
Positive regulation of epithelial cell proliferation Source: UniProtKB
Positive regulation of inflammatory response to wounding Source: UniProtKB
Positive regulation of lysosome organization Source: UniProtKB
Positive regulation of neuron apoptotic process Source: UniProtKB
Positive regulation of protein folding Source: UniProtKB
Protein stabilization Source: UniProtKB
Regulation of inflammatory response Source: GO_Central
Signal transduction Source: ProtInc

Cellular Location

Secreted; Lysosome. Endocytosed by SORT1 and delivred to lysosomes (PubMed:21092856, PubMed:28073925). Targeted to lysosome by PSAP via M6PR and LRP1, in both biosynthetic and endocytic pathways (PubMed:26370502, PubMed:28073925). Co-localized with GBA in the intracellular trafficking compartments until to lysosome (By similarity).

Involvement in disease

Ubiquitin-positive frontotemporal dementia (UP-FTD):
Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease.
Ceroid lipofuscinosis, neuronal, 11 (CLN11):
A form of neuronal ceroid lipofuscinosis characterized by rapidly progressive visual loss due to retinal dystrophy, seizures, cerebellar ataxia, and cerebellar atrophy. Cognitive decline may also occur. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material.

PTM

Cleaved by ELANE; proteolysis is blocked by SLPI and is concentration- and time-dependent and induces CXCL8/IL-8 production; granulin-3 and granulin-4 are resistant to ELANE (PubMed:12526812, PubMed:28743268). Cleaved by CTSL in lysosome thus regulating the maturation and turnover of progranulin within the lysosome (PubMed:28743268).

Boland, S., Swarup, S., Ambaw, Y. A., Malia, P. C., Richards, R. C., Fischer, A. W., ... & Farese Jr, R. V. (2022). Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis. Nature Communications, 13(1), 5924.

Zhou, X., Kukar, T., & Rademakers, R. (2021). Lysosomal dysfunction and other pathomechanisms in FTLD: evidence from progranulin genetics and biology. Frontotemporal Dementias: Emerging Milestones of the 21st Century, 219-242.

Petkau, T. L., Life, B., Lu, G., Yang, J., Fornes, O., Wasserman, W., ... & Leavitt, B. R. (2021). Human progranulin-expressing mice as a novel tool for the development of progranulin-modulating therapeutics. Neurobiology of Disease, 153, 105314.

Boland, S., Swarup, S., Ambaw, Y. A., Richards, R. C., Fischer, A. W., Singh, S., ... & Farese Jr, R. V. (2021). Progranulin deficiency results in reduced bis (monoacylglycero) phosphate (BMP) levels and gangliosidosis. bioRxiv, 2021-09.

Klupp, F., Kahlert, C., Franz, C., Halama, N., Schleussner, N., Wirsik, N. M., ... & Ulrich, A. B. (2021). Granulin: an invasive and survival-determining marker in colorectal cancer patients. International Journal of Molecular Sciences, 22(12), 6436.

Bhopatkar, A. A., Uversky, V. N., & Rangachari, V. (2020). Granulins modulate liquid–liquid phase separation and aggregation of the prion-like C-terminal domain of the neurodegeneration-associated protein TDP-43. Journal of Biological Chemistry, 295(8), 2506-2519.

Bateman, A., Cheung, S. T., & Bennett, H. P. (2018). A brief overview of progranulin in health and disease. Progranulin: Methods and Protocols, 3-15.

Paushter, D. H., Du, H., Feng, T., & Hu, F. (2018). The lysosomal function of progranulin, a guardian against neurodegeneration. Acta neuropathologica, 136, 1-17.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant GRN protein, His Tag (CAT#: V2LY-0526-LY6334)

GRN Matched Antibody Pair (501) (CAT#: APMAB-501LY)

GRN Matched Antibody Pair (502) (CAT#: APMAB-502LY)

Antibody Pairs

GRN Matched Antibody Pair (501) (CAT#: APMAB-501LY)

GRN Matched Antibody Pair (502) (CAT#: APMAB-502LY)

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GRN Matched Antibody Pair (502) (CAT#: APMAB-502LY)

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Human Recombinant GRN protein, His Tag (CAT#: V2LY-0526-LY6334)

Rabbit Anti-GRN Recombinant Antibody (BA0152) (CAT#: CBMAB-0360CQ)

Rat Anti-GRN Recombinant Antibody (21) (CAT#: CBMAB-1337-YC)

Human Anti-GRN Recombinant Antibody (BA0406) (CAT#: CBMAB-0361CQ)

Mouse Anti-GRN Recombinant Antibody (CBLG1-1807) (CAT#: V2LY-0226-LY1435)

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Isotype control

For research use only. Not intended for any clinical use.

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