Mouse Recombinant TRX protein (V2LY-0526-LY8966)

Name: Mouse Recombinant TRX protein
SKU: V2LY-0526-LY8966
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Expressed Host

E. coli

Protein Species

Mouse

Protein Construction

This product is Mouse Recombinant TRX protein consist of Amino Acid: 1-105 and predicts a molecular mass of 11.7 kDa.

Molecule Mass

11.7 kDa

Sequence

Amino Acid: 1-105

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

TXN Gene(Protein Coding) Thioredoxin

Function

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (PubMed:2176490, PubMed:17182577, PubMed:19032234).
Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity (PubMed:16408020, PubMed:17606900).
Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (PubMed:9108029, PubMed:11118054).
ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).

Biological Process

Biological Process activation of protein kinase B activity Source:ParkinsonsUK-UCL1 Publication
Biological Process cell redox homeostasis Source:UniProtKB1 Publication
Biological Process negative regulation of hydrogen peroxide-induced cell death Source:ParkinsonsUK-UCL1 Publication
Biological Process negative regulation of protein export from nucleus Source:Ensembl
Biological Process negative regulation of transcription by RNA polymerase II Source:Ensembl
Biological Process positive regulation of DNA binding Source:UniProtKB1 Publication
Biological Process positive regulation of peptidyl-cysteine S-nitrosylation Source:UniProtKB3 Publications
Biological Process positive regulation of peptidyl-serine phosphorylation Source:ParkinsonsUK-UCL1 Publication
Biological Process positive regulation of protein kinase B signaling Source:ParkinsonsUK-UCL1 Publication
Biological Process response to nitric oxide Source:UniProtKB2 Publications
Biological Process response to radiation Source:UniProtKB2 Publications

Cellular Location

Nucleus
Cytoplasm
Secreted
Translocates from the cytoplasm into the nucleus after phorbol 12-myristate 13-acetate induction (PMA) (PubMed:9108029).
Predominantly in the cytoplasm in non irradiated cells (PubMed:11118054).
Radiation induces translocation of TRX from the cytoplasm to the nucleus (PubMed:11118054).
Secreted by a leaderless secretory pathway (PubMed:1332947).

Involvement in disease

Causes an allergic reaction in human (PubMed:17182577, PubMed:19032234, PubMed:21489611).
Recombinant protein binds to IgE in atopic eczema-suffering patients allergic to opportunistic skin-colonizing yeast M.sympodialis. Intermediate cross-reactivity is detected between the recombinant thioredoxin from M.sympodialis (Mala s 13) and recombinant form of this protein. Skin-prick test (SPT) and atopy patch test (APT) with 5 patients confirm cross-reactivity between the two proteins (PubMed:17182577).
Recombinant protein binds to IgE of patients suffering from allergic bronchopulmonary aspergillosis (ABPA) and cross-reacts extensively with recombinant thioredoxin proteins from A.fumigatus (Asp f 28 and Asp f 29) and Mala s 13. Causes a positive skin reaction and induces proliferation of the human peripheral blood mononuclear cells in ABPA patients allergic to this protein. Acts as an IgE-binding self-antigen in ABPA patients allergic to fungal thioredoxin (PubMed:19032234).
In atopic dermatitis (AD)-suffering patients allergic to M.sympodialis, a cross-reactivity between Mala s 13 and this protein can be detected in T-cells of the peripheral blood and skin. Keratinocytes stimulated by interferon (IFN)-alpha and tumor necrosis factor (TNF)-alpha release thioredoxin, which then becomes available for cross-reactivity with Mala s 13-specific T cells. The autoreactive T cells identified include T-helper 1 (Th1), T-helper 2 (Th2), T-helper 17 (Th17) and T-helper 22 (Th22) phenotypes. Skinhoming T cells autoreactive to this protein may be relevant for cutaneous inflammation in patients with AD (PubMed:21489611).

PTM

In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant TXN protein (CAT#: V2LY-0526-LY7194)

Human TXN ELISA Kit (CAT#: V2LY-0626-LY1667)

Cattle TXN ELISA Kit (CAT#: V2LY-0626-LY2370)

Mouse Txn ELISA Kit (CAT#: V2LY-0626-LY435)

Rabbit TXN ELISA Kit (CAT#: V2LY-0626-LY458)

Rat Txn ELISA Kit (CAT#: V2LY-0626-LY884)

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Isotype control

For research use only. Not intended for any clinical use.

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