Mouse Recombinant VCL protein, His Tag (V2LY-0526-LY9066)

Name: Mouse Recombinant VCL protein, His Tag
SKU: V2LY-0526-LY9066
Rating: 5 (1 reviews)

See all VCL antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant VCL protein, His Tag consist of Amino Acid: 1-1066 and predicts a molecular mass of 118.2 kDa.

Molecule Mass

118.2 kDa

Sequence

Amino Acid: 1-1066

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Vinculin

Function

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Biological Process

Biological Process adherens junction assembly Source:BHF-UCL1 Publication
Biological Process apical junction assembly Source:UniProtKB1 Publication
Biological Process axon extension Source:Ensembl
Biological Process cell adhesion Source:GO_Central1 Publication
Biological Process cell-matrix adhesion Source:BHF-UCL1 Publication
Biological Process epithelial cell-cell adhesion Source:BHF-UCL1 Publication
Biological Process lamellipodium assembly Source:UniProtKB
Biological Process maintenance of blood-brain barrier Source:ARUK-UCL1 Publication
Biological Process morphogenesis of an epithelium Source:BHF-UCL1 Publication
Biological Process negative regulation of cell migration Source:UniProtKB1 Publication
Biological Process platelet aggregation Source:UniProtKB1 Publication
Biological Process protein localization to cell surface Source:BHF-UCL1 Publication
Biological Process regulation of establishment of endothelial barrier Source:ARUK-UCL1 Publication
Biological Process regulation of focal adhesion assembly Source:ARUK-UCL1 Publication
Biological Process regulation of protein localization to adherens junction Source:ARUK-UCL1 Publication

Cellular Location

Cell membrane
Cell junction, adherens junction
Cell junction, focal adhesion
Cytoplasm, cytoskeleton
Cell membrane, sarcolemma
Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions.

Involvement in disease

Cardiomyopathy, dilated 1W (CMD1W):
A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Cardiomyopathy, familial hypertrophic 15 (CMH15):
A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.

PTM

Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques (By similarity).
Acetylated; mainly by myristic acid but also by a small amount of palmitic acid.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Recombinant VCL protein, His Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-VCL Recombinant Antibody (CBFYM-3179) (CAT#: CBMAB-M3376-FY)

Rabbit Anti-VCL Recombinant Antibody (CBYY-1375) (CAT#: CBMAB-1417-YY)

Rabbit Anti-VCL Recombinant Antibody (E1E9V) (CAT#: CBMAB-V0035-LY)

Mouse Anti-VCL Recombinant Antibody (7F9) (CAT#: CBMAB-V0027-LY)

Mouse Anti-VCL Recombinant Antibody (CBYY-1373) (CAT#: CBMAB-1415-YY)

Mouse Anti-VCL Recombinant Antibody (CBYY-1371) (CAT#: CBMAB-1413-YY)

Mouse Anti-VCL Recombinant Antibody (VLN01) (CAT#: CBMAB-V0285-LY)

Rabbit Anti-VCL Recombinant Antibody (SP117) (CAT#: CBMAB-V0261-LY)

Mouse Anti-VCL Recombinant Antibody (FB11) (CAT#: CBMAB-V0152-LY)

Mouse Anti-VCL Recombinant Antibody (10g74) (CAT#: CBMAB-V0123-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry