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Human NFκB Pathway Phosphorylation Antibody Array (Membrane, 11 Targets) (Y0563) (AbAr-0563-YC)


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Specifications

Target Array
NFκB Pathway Phosphorylation Array
Target
ATM (pSer1981), eIF-2a (pSer52), HDAC2 (pSer394), HDAC4 (pSer632), IKB-alpha (pSer32), MSK1 (pSer376), NF-kB (pSer536), STAT1 (pSer727), TAK1 (pSer412), TBK1 (pSer172), ZAP70 (pTyr292)
Species Reactivity
Human
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based Assay
Protocol Outline
Perform blocking of the array membrane surface.
Incubate with the sample.
Incubate using the detection antibody cocktail.
Proceed with incubation using HRP-anti-rabbit IgG antibodies.
Engage in incubation with detection buffers.
Acquire images with a chemiluminescent imaging system.
Conduct a comprehensive data analysis.
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction
A neuron, also known as a neurone or nerve cell, is an electrically excitable cell that communicates with other cells via specialized connections called synapses. It is the main component of nervous tissue. All animals except sponges and placozoans have neurons, but other multicellular organisms such as plants do not.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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