Anti-NKX2-1 Antibody (V2LY-0826-LY30) Immunohistochemistry Kit (V2LY-0826-LY146)

Name: Anti-NKX2-1 Antibody (V2LY-0826-LY30) Immunohistochemistry Kit
SKU: V2LY-0826-LY146
Rating: 5 (1 reviews)

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Summary

Product Overview

This comprehensive, all-in-one kit is designed for the precise immunohistochemical (IHC) detection of target protein in tissue samples. Unlike standard detection kits, it includes a highly specific mouse primary antibody bundled directly with our robust Polymer HRP detection system. The kit provides every essential reagent needed for the entire IHC workflow-from the initial antigen retrieval buffer to the final mounting media. By offering a pre-matched primary antibody with optimized buffers and secondary detection reagents, this kit eliminates the need for tedious protocol optimization and reagent sourcing, ensuring consistent, high-quality staining results.

Primary Antibody Cat.

V2LY-0826-LY30

Primary Antibody

Mouse Anti-NKX2-1 Recombinant Antibody (CBWJN-0756)

Secondary Antibody Host Species

Goat

Secondary Antibody Species Specificity

Mouse

Application

IHC-P

Sample Type

FFPE tissue

Test Size

50 Tests

Storage

Stored at 2-8°C. The kit is stable for 6 months from the date of receipt.

Key Features

Target-Specific Primary Antibody

Includes a highly validated mouse primary antibody, ensuring reliable and specific target localization.

Complete End-to-End Solution

Contains all 11 essential components required for a full IHC workflow, including Tris-EDTA Antigen Retrieval Buffer, Blocking Buffer, Polymer HRP-Goat anti-Mouse Secondary Antibody, DAB Chromogens, Counterstain, and Mounting Media.

Streamlined Workflow

Pre-optimized reagents work seamlessly with the included primary antibody, saving researchers significant time on antibody titration and protocol adjustments.

Sample Compatibility

Highly recommended and optimized for Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.

Kit Capacity

Provides sufficient reagents (50T) to perform 50 tests, depending on tissue section size.

More Infomation

Sub Cat	Reagents	Size
LV01	Antigen Retrieval Buffer (50× Tris-EDTA)	100 ml
LV02	Washing Buffer (30×)	120 ml
LV03	Blocking Buffer	5 ml
LV04	Quenching buffer (RTU)	5 ml
LV05	Primary Antibody Diluent (RTU)	100 ml
LV06M	HRP-Goat anti-Mouse Secondary Antibody	5 ml
LV07	Chromogen Component A	0.2 ml
LV08	Chromogen Component B	4 ml
LV09	Signal Enhancer	5 ml
LV10	Counter Staining Reagent	5 ml
LV11	Mounting Media	5 ml

Soreze, Y., Nathan, N., Jegard, J., Hervieux, E., Clermidi, P., Sileo, C., ... & Coulomb L'Herminé, A. (2024). Acinar dysplasia in a full-term newborn with a NKX2. 1 variant. Neonatology, 121(1), 133-136.

Heylen, E., Verstraete, P., Van Aerschot, L., Geeraerts, S. L., Venken, T., Timcheva, K., ... & Kampen, K. R. (2023). Transcription factor NKX2–1 drives serine and glycine synthesis addiction in cancer. British Journal of Cancer, 128(10), 1862-1878.

López, J. M., Jiménez, S., Morona, R., Lozano, D., & Moreno, N. (2022). Analysis of Islet‐1, Nkx2. 1, Pax6, and Orthopedia in the forebrain of the sturgeon Acipenser ruthenus identifies conserved prosomeric characteristics. Journal of Comparative Neurology, 530(5), 834-855.

Teng, F., Zhang, J. X., Chen, Y., Shen, X. D., Su, C., Guo, Y. J., ... & Liu, S. Q. (2021). LncRNA NKX2‐1‐AS1 promotes tumor progression and angiogenesis via upregulation of SERPINE1 expression and activation of the VEGFR‐2 signaling pathway in gastric cancer. Molecular oncology, 15(4), 1234-1255.

Stuart, W. D., Fink-Baldauf, I. M., Tomoshige, K., Guo, M., & Maeda, Y. (2021). CRISPRi-mediated functional analysis of NKX2-1-binding sites in the lung. Communications Biology, 4(1), 568.

Valero, M., Viney, T. J., Machold, R., Mederos, S., Zutshi, I., Schuman, B., ... & Buzsáki, G. (2021). Sleep down state-active ID2/Nkx2. 1 interneurons in the neocortex. Nature neuroscience, 24(3), 401-411.

Little, D. R., Lynch, A. M., Yan, Y., Akiyama, H., Kimura, S., & Chen, J. (2021). Differential chromatin binding of the lung lineage transcription factor NKX2-1 resolves opposing murine alveolar cell fates in vivo. Nature communications, 12(1), 2509.

Abecassis, Z. A., Berceau, B. L., Win, P. H., Garcia, D., Xenias, H. S., Cui, Q., ... & Chan, C. S. (2020). Npas1+-Nkx2. 1+ neurons are an integral part of the cortico-pallido-cortical loop. Journal of Neuroscience, 40(4), 743-768.

Murcia-Ramón, R., Company, V., Juárez-Leal, I., Andreu-Cervera, A., Almagro-García, F., Martínez, S., ... & Puelles, E. (2020). Neuronal tangential migration from Nkx2. 1-positive hypothalamus. Brain Structure and Function, 225, 2857-2869.

Orquera, D. P., Tavella, M. B., de Souza, F. S., Nasif, S., Low, M. J., & Rubinstein, M. (2019). The homeodomain transcription factor NKX2. 1 is essential for the early specification of melanocortin neuron identity and activates Pomc expression in the developing hypothalamus. Journal of Neuroscience, 39(21), 4023-4035.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Nkx2-1 ELISA Kit (CAT#: V2LY-0626-LY1643)

Human NKX2-1 ELISA Kit (CAT#: V2LY-0626-LY3748)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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