Pig EGF ELISA Kit (V2LY-0626-LY959)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.098 ng/mL
Detection Range
0.2-20 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Pig
Assay Type
Sandwich
Reactivity
Pig
Assay Time
1.5 h
Molecule Mass
133.5 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Epidermal Growth Factor
Research Area
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
Biological Process
Angiogenesis Source: HGNC-UCL
Branching morphogenesis of an epithelial tube Source: Ensembl
Epidermal growth factor receptor signaling pathway Source: BHF-UCL
Epidermal growth factor receptor signaling pathway via MAPK cascade Source: UniProtKB
ERK1 and ERK2 cascade Source: UniProtKB
Mammary gland alveolus development Source: Ensembl
Negative regulation of cholesterol efflux Source: Ensembl
Negative regulation of secretion Source: BHF-UCL
Peptidyl-tyrosine phosphorylation Source: Ensembl
Positive regulation of canonical Wnt signaling pathway Source: BHF-UCL
Positive regulation of cell migration Source: UniProtKB
Positive regulation of cell population proliferation Source: HGNC-UCL
Positive regulation of cerebellar granule cell precursor proliferation Source: Ensembl
Positive regulation of DNA binding Source: UniProtKB
Positive regulation of DNA replication Source: UniProtKB
Positive regulation of endothelial cell migration Source: BHF-UCL
Positive regulation of endothelial cell proliferation Source: BHF-UCL
Positive regulation of epidermal growth factor-activated receptor activity Source: UniProtKB
Positive regulation of epithelial tube formation Source: BHF-UCL
Positive regulation of gene expression Source: UniProtKB
Positive regulation of hyaluronan biosynthetic process Source: UniProtKB
Positive regulation of MAPK cascade Source: BHF-UCL
Positive regulation of MAP kinase activity Source: HGNC-UCL
Positive regulation of mitotic nuclear division Source: HGNC-UCL
Positive regulation of peptidyl-threonine phosphorylation Source: UniProtKB
Positive regulation of phosphatidylinositol 3-kinase signaling Source: BHF-UCL
Positive regulation of phosphorylation Source: HGNC-UCL
Positive regulation of protein kinase B signaling Source: BHF-UCL
Positive regulation of protein localization to early endosome Source: UniProtKB
Positive regulation of receptor internalization Source: ParkinsonsUK-UCL
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of ubiquitin-dependent protein catabolic process Source: Ensembl
Regulation of calcium ion import Source: BHF-UCL
Regulation of protein localization to cell surface Source: BHF-UCL
Regulation of receptor signaling pathway via JAK-STAT Source: UniProtKB
Cellular Location
Membrane
Involvement in disease
Hypomagnesemia 4 (HOMG4):
A disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to moderate psychomotor retardation, and brisk tendon reflexes.
Topology
Extracellular: 23-1032
Helical: 1033-1053
Cytoplasmic: 1054-1207
PTM
O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).

Yasar, Z., Elliott, B. T., Kyriakidou, Y., Nwokoma, C. T., Postlethwaite, R. D., Gaffney, C. J., ... & Hayes, L. D. (2021). Sprint interval training (SIT) reduces serum epidermal growth factor (EGF), but not other inflammatory cytokines in trained older men. European Journal of Applied Physiology, 121(7), 1909-1919.

Wang, L., Zhu, F., Yang, H., Li, J., Li, Y., Ding, X., ... & Yin, Y. (2019). Effects of dietary supplementation with epidermal growth factor on nutrient digestibility, intestinal development and expression of nutrient transporters in early‐weaned piglets. Journal of animal physiology and animal nutrition, 103(2), 618-625.

Nan, Y. (2019). Lung carcinoma therapy using epidermal growth factor receptor‑targeted lipid polymeric nanoparticles co‑loaded with cisplatin and doxorubicin. Oncology reports, 42(5), 2087-2096.

Zhang, J., Hu, W., Diao, Q., Wang, Z., Miao, J., Chen, X., & Xue, Z. (2019). Therapeutic effect of the epidermal growth factor on diabetic foot ulcer and the underlying mechanisms. Experimental and therapeutic medicine, 17(3), 1643-1648.

Macdonald-Obermann, J. L., & Pike, L. J. (2018). Allosteric regulation of epidermal growth factor (EGF) receptor ligand binding by tyrosine kinase inhibitors. Journal of Biological Chemistry, 293(35), 13401-13414.

Richani, D., & Gilchrist, R. B. (2018). The epidermal growth factor network: role in oocyte growth, maturation and developmental competence. Human reproduction update, 24(1), 1-14.

Dao, D. T., Anez-Bustillos, L., Adam, R. M., Puder, M., & Bielenberg, D. R. (2018). Heparin-binding epidermal growth factor–like growth factor as a critical mediator of tissue repair and regeneration. The American journal of pathology, 188(11), 2446-2456.

Vieceli Dalla Sega, F., Fortini, F., Aquila, G., Pavasini, R., Biscaglia, S., Bernucci, D., ... & Campo, G. (2018). Ticagrelor improves endothelial function by decreasing circulating epidermal growth factor (EGF). Frontiers in physiology, 9, 337.

Jia, Y. Y., Zhou, J. Y., Chang, Y., An, F., Li, X. W., Xu, X. Y., ... & Wang, J. L. (2018). Effect of optimized concentrations of basic fibroblast growth factor and epidermal growth factor on proliferation of fibroblasts and expression of collagen: related to pelvic floor tissue regeneration. Chinese Medical Journal, 131(17), 2089-2096.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rabbit EGF ELISA Kit (CAT#: V2LY-0626-LY179)

Human EGF ELISA Kit (CAT#: V2LY-0626-LY468)

Canine Recombinant EGF protein (CAT#: V2LY-0526-LY1234)

Canine Recombinant EGF protein, His Tag (CAT#: V2LY-0526-LY1235)

Feline Recombinant EGF protein, ECD, hFc Tag (CAT#: V2LY-0526-LY1785)

Human Recombinant EGF proteinECD (CAT#: V2LY-0526-LY3693)

Human Recombinant EGF proteinECD-1 (CAT#: V2LY-0526-LY3694)

Human Recombinant EGF protein, ECD, hFc Tag (CAT#: V2LY-0526-LY3695)

Human Recombinant EGF protein, ECD, hFc Tag-1 (CAT#: V2LY-0526-LY3696)

Mouse Recombinant EGF protein (CAT#: V2LY-0526-LY8219)

Related Products

Mouse Anti-EGF Recombinant Antibody (CBFYE-0290) (CAT#: V2LY-0425-LY1530)

Rabbit EGF ELISA Kit (CAT#: V2LY-0626-LY179)

Rabbit Anti-EGF Recombinant Antibody (CBFYE-0523) (CAT#: V2LY-0425-LY1537)

Canine Recombinant EGF protein, His Tag (CAT#: V2LY-0526-LY1235)

Human EGF ELISA Kit (CAT#: V2LY-0626-LY468)

Human Recombinant EGF protein, ECD, hFc Tag-1 (CAT#: V2LY-0526-LY3696)

Rat Anti-EGF Recombinant Antibody (CBFYE-0512) (CAT#: V2LY-0425-LY1531)

Feline Recombinant EGF protein, ECD, hFc Tag (CAT#: V2LY-0526-LY1785)

Human Recombinant EGF protein, ECD, hFc Tag (CAT#: V2LY-0526-LY3695)

Mouse Recombinant EGF protein, hFc Tag (CAT#: V2LY-0526-LY8221)

Isotype control

For research use only. Not intended for any clinical use.

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