Mouse Anti-Pirh2/RCHY1 Recombinant Antibody (7B9-H8-D2) (CBMAB-Z0213-LY)

See all Pirh2/RCHY1 antibodies
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Basic Information

Host Animal
Mouse
Clone
7B9-H8-D2
Application
WB, IHC, ICC, FC
Immunogen
Purified recombinant fragment of human Pirh2 expressed in E. Coli.
Specificity
Human, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Pirh2/RCHY1
Entrez Gene ID
Human25898
Rat289508
UniProt ID
HumanQ96PM5
RatQ5XIE4
Alternative Names
ARNIP; CHIMP; RNF199; RCHY1
Function
Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, P73, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Monoubiquitinates the translesion DNA polymerase POLH. Contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity.
Biological Process
Error-free translesion synthesisTAS:Reactome
Positive regulation of proteasomal ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of protein ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Protein autoubiquitinationIDA:UniProtKB
Protein ubiquitinationManual Assertion Based On ExperimentIDA:UniProtKB
Ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIDA:UniProtKB
Cellular Location
Nucleus
Nucleus speckle
Cytoplasm
PTM
Subject to ubiquitination and proteasomal degradation. Interaction with PLAGL2 or KAT5 enhances protein stability.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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