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Rabbit Anti-Acetyl-Histone H4 (Lys16) Recombinant Antibody (V2-623415) (CBMAB-CP1021-LY)


See all Acetyl-Histone H4 (Lys16) antibodies
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Published Data
Rabbit Anti-Acetyl-Histone H4 (Lys16) Recombinant Antibody (E2B8W) in CIP
Representative genome tracks of Pol II, p300, Brd4, and NPS binding, Omni-ATAC and histone acetylation across core histones in WT and MZnps embryos. ...View More
Miao, L., Tang, Y., Bonneau, A. R., Chan, S. H., Kojima, M. L., Pownall, M. E., ... & Giraldez, A. J. (2022). The landscape of pioneer factor activity reveals the mechanisms of chromatin reprogramming and genome activation. Molecular Cell, 82(5), 986-1002.
Rabbit Anti-Acetyl-Histone H4 (Lys16) Recombinant Antibody (E2B8W) in CIP
Acetylated H4K16 (H4K16ac levels at the CXCR4 promoter in vehicle or Flonase treated human CB CD34+ cells, as assessed by a ChIP assay. Data pooled from two independent experiments are shown (n=8 replicates per group). ...View More
Guo, B., Huang, X., Cooper, S., & Broxmeyer, H. E. (2017). Glucocorticoid hormone-induced chromatin remodeling enhances human hematopoietic stem cell homing and engraftment. Nature medicine, 23(4), 424-428.
Rabbit Anti-Acetyl-Histone H4 (Lys16) Recombinant Antibody (E2B8W) in IF
Immunodetection of H4K16ac in the indicated TDF cells 14 d after knock-out, with percentages of H4K16aclow cells indicated. Scale bar: 10 μm. ...View More
Monserrat, J., Morales Torres, C., Richardson, L., Wilson, T. S., Patel, H., Domart, M. C., ... & Scaffidi, P. (2021). Disruption of the MSL complex inhibits tumour maintenance by exacerbating chromosomal instability. Nature cell biology, 23(4), 401-412.
Rabbit Anti-Acetyl-Histone H4 (Lys16) Recombinant Antibody (E2B8W) in IF
HFs were treated with TSA (1 μM) and NAM (1 mM) for 4 h. Histone 4 acetylated on lysine 16 (Ac-H4K16) (green) was detected by immunofluorescence, and the nuclei were stained with Hoechst 33342 (blue). Original magnification, × 40; scale bar corresponds to 10 μm. ...View More
Yakhine-Diop, S. M., Niso-Santano, M., Rodríguez-Arribas, M., Gómez-Sánchez, R., Martínez-Chacón, G., Uribe-Carretero, E., ... & Fuentes, J. M. (2019). Impaired mitophagy and protein acetylation levels in fibroblasts from Parkinson's disease patients. Molecular neurobiology, 56, 2466-2481.
Rabbit Anti-Acetyl-Histone H4 (Lys16) Recombinant Antibody (E2B8W) in WB
Cis-RSV and trans-RSV have opposite effects on the auto-PARylation of PARP1. Representative immunoblot images and quantification using specific antibodies for PARylation, PARP1, AcK16-H4, AcK56-H3 levels after treatment of cortical neurons (DIV 9) with cis- and trans-RSV for 15 min. ...View More
Jhanji, M., Rao, C. N., Massey, J. C., Hope III, M. C., Zhou, X., Keene, C. D., ... & Sajish, M. (2022). Cis-and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration. Nature Communications, 13(1), 3244.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Clone
V2-623415
Antibody Isotype
IgG
Application
WB, IP, IF (ICC), IHC-F, CI

Basic Information

Immunogen
Synthetic peptide corresponding to residues surrounding acetylated Lys16 of human histone H4 protein.
Host Species
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IF(ICC)1:1,600
FC1:800
IP1:50
CIP1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES pH 7.5, 0.01% BSA, 50% Glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Acetyl-Histone H4 (Lys16)
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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