Rat Ggt1 ELISA Kit (V2LY-0626-LY1472)

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Tested Data
Datasheet
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Basic Information

Sensitivity
1.13 mIU/mL
Detection Range
2-600 mIU/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Rat
Assay Type
Sandwich
Reactivity
Rat
Assay Time
1.5 h
Molecule Mass
61.6 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Gamma-Glutamyltransferase 1
Function
Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates and other gamma-glutamyl compounds, such as leukotriene C4 (LTC4). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound (PubMed:17924658, PubMed:7673200, PubMed:7759490, PubMed:8095045, PubMed:8827453, PubMed:21447318).

Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4.

Isoform 3:
Seems to be inactive.
Biological Process
Cellular amino acid metabolic process Source: UniProtKB
Cysteine biosynthetic process Source: UniProtKB
Fatty acid metabolic process Source: BHF-UCL
Glutamate metabolic process Source: UniProtKB
Glutathione biosynthetic process Source: UniProtKB
Glutathione catabolic process Source: UniProtKB
Leukotriene D4 biosynthetic process Source: BHF-UCL
Leukotriene metabolic process Source: UniProtKB
Peptide modification Source: GO_Central
Proteolysis Source: UniProtKB
Regulation of immune system process Source: UniProtKB
Regulation of inflammatory response Source: UniProtKB
Response to estradiol Source: GO_Central
Response to lipopolysaccharide Source: GO_Central
Response to tumor necrosis factor Source: GO_Central
Spermatogenesis Source: UniProtKB
Zymogen activation Source: UniProtKB
Cellular Location
Cell membrane
Involvement in disease
Glutathionuria (GLUTH):
A very rare, autosomal recessive metabolic disorder characterized by the presence of glutathione in the urine, due to generalized gamma-glutamyl transpeptidase deficiency. Most patients manifest mild to moderate mental retardation, and behavioral disturbance. Seizures, tremor, marfanoid features and strabismus are observed in some patients.
Topology
Cytoplasmic: 1-4
Helical: 5-26
Extracellular: 27-569
PTM
N-glycosylated on both chains. Contains hexoses, hexosamines and sialic acid residues. Glycosylation profiles tested in kidney and liver tissues reveal the presence of tissue-specific and site-specific glycan composition, despite the overlap in composition among the N-glycans. A total of 36 glycan compositions, with 40 unique structures are observed. Up to 15 different glycans are observed at a single site, with site-specific variation in glycan composition. The difference in glycosylation profiles in the 2 tissues do not affect the enzyme activity.
Cleaved by autocatalysis into a large and a small subunit and the autocatalytic cleavage is essential to the functional activation of the enzyme.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Ggt1 ELISA Kit (CAT#: V2LY-0626-LY5801)

Human Recombinant GGT1 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY4302)

Human Recombinant GGT1 protein, ECD, His Tag (CAT#: V2LY-0526-LY4303)

Rat Recombinant GGT1 protein, His Tag (CAT#: V2LY-0526-LY9394)

GGT1 Matched Antibody Pair (482) (CAT#: APMAB-482LY)

Antibody Pairs

GGT1 Matched Antibody Pair (482) (CAT#: APMAB-482LY)

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Mouse Ggt1 ELISA Kit (CAT#: V2LY-0626-LY5801)

Mouse Anti-GGT1 Recombinant Antibody (CBFYH-0340) (CAT#: V2LY-1225-LY1176)

Human Recombinant GGT1 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY4302)

Mouse Anti-GGT1 Recombinant Antibody (CBLG1-1058) (CAT#: CBMAB-G3275-LY)

Mouse Anti-GGT1 Recombinant Antibody (CBLG1-1060) (CAT#: V2LY-1225-LY1181)

Mouse Anti-GGT1 Recombinant Antibody (CBLG1-1058) (CAT#: V2LY-1225-LY1179)

Mouse Anti-GGT1 Recombinant Antibody (CAP835) (CAT#: CBMAB-AP2522LY)

Human Recombinant GGT1 protein, ECD, His Tag (CAT#: V2LY-0526-LY4303)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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