Rat Hmgcr ELISA Kit (V2LY-0626-LY2020)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.048 ng/mL
Detection Range
0.1-40 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Rat
Assay Type
Sandwich
Reactivity
Rat
Assay Time
1.5 h
Molecule Mass
96.7 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
3-Hydroxy-3-Methylglutaryl-CoA Reductase
Function
Catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis (PubMed:2991281, PubMed:21357570, PubMed:6995544).

HMGCR is the main target of statins, a class of cholesterol-lowering drugs (PubMed:11349148, PubMed:18540668).
Biological Process
Cholesterol biosynthetic process Source: CACAO
Coenzyme A metabolic process Source: InterPro
Isoprenoid biosynthetic process Source: GO_Central
Negative regulation of amyloid-beta clearance Source: ARUK-UCL
Negative regulation of MAP kinase activity Source: Ensembl
Negative regulation of protein catabolic process Source: ARUK-UCL
Negative regulation of protein secretion Source: ARUK-UCL
Sterol biosynthetic process Source: GO_Central
Visual learning Source: Ensembl
Cellular Location
Peroxisome membrane; Endoplasmic reticulum membrane
Topology
Cytoplasmic: 1-9
Helical: 10-39
Lumenal: 40-56
Helical: 57-78
Cytoplasmic: 79-89
Helical: 90-114
Lumenal: 115-123
Helical: 124-149
Cytoplasmic: 150-159
Helical: 160-187
Lumenal: 188-191
Helical: 192-220
Cytoplasmic: 221-248
Helical: 249-275
Lumenal: 276-314
Helical: 315-339
Cytoplasmic: 340-888
PTM
N-glycosylated. Deglycosylated by NGLY1 on release from the endoplasmic reticulum (ER) in a sterol-mediated manner.
Undergoes sterol-mediated ubiquitination and ER-associated degradation (ERAD) (PubMed:12535518, PubMed:19458199, PubMed:21778231). Accumulation of sterols in the endoplasmic reticulum (ER) membrane, triggers binding of the reductase to the ER membrane protein INSIG1 or INSIG2 (PubMed:12535518, PubMed:19458199, PubMed:21778231, PubMed:22143767). The INSIG1 binding leads to the recruitment of the ubiquitin ligase, AMFR/gp78, RNF139 or RNF145, initiating ubiquitination of the reductase (PubMed:12535518, PubMed:19458199, PubMed:21778231). The ubiquitinated reductase is then extracted from the ER membrane and delivered to cytosolic 26S proteosomes by a mechanism probably mediated by the ATPase Valosin-containing protein VCP/p97 (PubMed:12535518, PubMed:19458199, PubMed:21778231). The INSIG2-binding leads to the recruitment of the ubiquitin ligase RNF139, initiating ubiquitination of the reductase (PubMed:22143767). Lys-248 is the main site of ubiquitination (PubMed:19458199). Ubiquitination is enhanced by the presence of a geranylgeranylated protein (PubMed:21778231).
Phosphorylated. Phosphorylation at Ser-872 reduces the catalytic activity.

Kurashige, T. (2021). Anti-HMGCR myopathy: clinical and histopathological features, and prognosis. Current Opinion in Rheumatology, 33(6), 554-562.

Huang, J., Zhao, X., Li, X., Peng, J., Yang, W., & Mi, S. (2021). HMGCR inhibition stabilizes the glycolytic enzyme PKM2 to support the growth of renal cell carcinoma. PLoS biology, 19(4), e3001197.

Lu, X. Y., Shi, X. J., Hu, A., Wang, J. Q., Ding, Y., Jiang, W., ... & Song, B. L. (2020). Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis. Nature, 588(7838), 479-484.

Meyer, A., Troyanov, Y., Drouin, J., Oligny-Longpré, G., Landon-Cardinal, O., Hoa, S., ... & Senécal, J. L. (2020). Statin-induced anti-HMGCR myopathy: successful therapeutic strategies for corticosteroid-free remission in 55 patients. Arthritis research & therapy, 22(1), 1-10.

Ma, S., Sun, W., Gao, L., & Liu, S. (2019). Therapeutic targets of hypercholesterolemia: HMGCR and LDLR. Diabetes, metabolic syndrome and obesity: targets and therapy, 1543-1553.

Mohassel, P., Landon-Cardinal, O., Foley, A. R., Donkervoort, S., Pak, K. S., Wahl, C., ... & Bönnemann, C. G. (2019). Anti-HMGCR myopathy may resemble limb-girdle muscular dystrophy. Neurology-Neuroimmunology Neuroinflammation, 6(1).

Bergua, C., Chiavelli, H., Allenbach, Y., Arouche-Delaperche, L., Arnoult, C., Bourdenet, G., ... & Boyer, O. (2019). In vivo pathogenicity of IgG from patients with anti-SRP or anti-HMGCR autoantibodies in immune-mediated necrotising myopathy. Annals of the rheumatic diseases, 78(1), 131-139.

Chen, L., Ma, M. Y., Sun, M., Jiang, L. Y., Zhao, X. T., Fang, X. X., ... & Song, B. L. (2019). Endogenous sterol intermediates of the mevalonate pathway regulate HMGCR degradation and SREBP-2 processing [S]. Journal of lipid research, 60(10), 1765-1775.

Landon-Cardinal, O., Allenbach, Y., Soulages, A., Rigolet, A., Hervier, B., Champtiaux, N., ... & Benveniste, O. (2019). Rituximab in the treatment of refractory anti-HMGCR immune-mediated necrotizing myopathy. The Journal of Rheumatology, 46(6), 623-627.

Mohassel, P., & Mammen, A. L. (2018). Anti-HMGCR myopathy. Journal of neuromuscular diseases, 5(1), 11-20.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant HMGCR protein, His Tag (CAT#: V2LY-0526-LY4563)

Human HMGCR ELISA Kit (CAT#: V2LY-0626-LY1294)

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For research use only. Not intended for any clinical use.

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