Rat Hspe1 ELISA Kit (V2LY-0626-LY3130)

Name: Rat Hspe1 ELISA Kit
SKU: V2LY-0626-LY3130
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.33 ng/mL

Detection Range

0.5-100 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Rat

Assay Type

Sandwich

Reactivity

Rat

Assay Time

1.5 h

Molecule Mass

10.9 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

heat shock 10kDa protein 1 (chaperonin 10)

Function

Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:7912672, PubMed:1346131, PubMed:11422376).

The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).

Biological Process

Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Chaperone cofactor-dependent protein refolding Source: GO_Central
Osteoblast differentiation Source: UniProtKB
Protein folding Source: ProtInc
Response to unfolded protein Source: ProtInc

Cellular Location

Mitochondrion matrix

Yeung, N., Murata, D., Iijima, M., & Sesaki, H. (2023). Role of human HSPE1 for OPA1 processing independent of HSPD1. Iscience, 26(2).

Boudreault, J., Wang, N., Yan, G., Dai, M., Poulet, S., Daliah, G., & Lebrun, J. J. (2023). In vivo genome-wide CRISPR screen in pancreatic ductal adenocarcinoma defines HSPE1 as a potential oncogene by acting through G2/M cell cycle arrest. Cancer Research, 83(7_Supplement), 3938-3938.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human HSPE1 ELISA Kit (CAT#: V2LY-0626-LY1073)

Human Recombinant HSPE1 protein, GST Tag (CAT#: V2LY-0526-LY3351)

Human Recombinant HSPE1 protein, His Tag (CAT#: V2LY-0526-LY3352)

Related Products

Human Recombinant HSPE1 protein, His Tag (CAT#: V2LY-0526-LY3352)

Mouse Anti-HSPE1 (AA 1-103) Monoclonal Antibody (CBFYH-3262) (CAT#: CBMAB-H3468-FY)

Mouse Anti-HSPE1 Monoclonal Antibody (CBFYH-3261) (CAT#: CBMAB-H0474-FY)

Mouse Anti-HSPE1 (AA 2-102) Recombinant Antibody (CBFYH-2453) (CAT#: CBMAB-H3470-FY)

Human Recombinant HSPE1 protein, GST Tag (CAT#: V2LY-0526-LY3351)

Mouse Anti-HSPE1 Recombinant Antibody (CBFYH-2452) (CAT#: CBMAB-H0440-FY)

Mouse Anti-HSPE1 Recombinant Antibody (4C11-B11) (CAT#: CBMAB-A4147-LY)

Human HSPE1 ELISA Kit (CAT#: V2LY-0626-LY1073)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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