Rat Recombinant F2 protein, His Tag (V2LY-0526-LY9303)

Name: Rat Recombinant F2 protein, His Tag
SKU: V2LY-0526-LY9303
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rat

Tag

His Tag

Protein Construction

This product is Rat Recombinant F2 protein, His Tag consist of Amino Acid: 1-617 and predicts a molecular mass of 69.5 kDa.

Molecule Mass

69.5 kDa

Sequence

Amino Acid: 1-617

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Coagulation Factor II, Thrombin

Research Area

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.

Biological Process

Acute-phase response Source: UniProtKB-KW
Antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
Blood coagulation Source: ProtInc
Cell surface receptor signaling pathway Source: BHF-UCL
Cytolysis by host of symbiont cells Source: UniProtKB
Fibrinolysis Source: UniProtKB
Multicellular organism development Source: ProtInc
Negative regulation of astrocyte differentiation Source: BHF-UCL
Negative regulation of cytokine production involved in inflammatory response Source: UniProtKB
Negative regulation of fibrinolysis Source: BHF-UCL
Negative regulation of platelet activation Source: BHF-UCL
Negative regulation of proteolysis Source: BHF-UCL
Neutrophil-mediated killing of gram-negative bacterium Source: UniProtKB
Platelet activation Source: BHF-UCL
Positive regulation of blood coagulation Source: BHF-UCL
Positive regulation of cell growth Source: Ensembl
Positive regulation of cell population proliferation Source: Ensembl
Positive regulation of collagen biosynthetic process Source: BHF-UCL
Positive regulation of lipid kinase activity Source: BHF-UCL
Positive regulation of phosphatidylinositol 3-kinase signaling Source: Ensembl
Positive regulation of phospholipase C-activating G protein-coupled receptor signaling pathway Source: BHF-UCL
Positive regulation of protein localization to nucleus Source: BHF-UCL
Positive regulation of protein phosphorylation Source: BHF-UCL
Positive regulation of reactive oxygen species metabolic process Source: UniProtKB
Positive regulation of receptor signaling pathway via JAK-STAT Source: BHF-UCL
Positive regulation of release of sequestered calcium ion into cytosol Source: BHF-UCL
Proteolysis Source: ProtInc
Regulation of blood coagulation Source: UniProtKB
Regulation of cell shape Source: Ensembl
Regulation of cytosolic calcium ion concentration Source: BHF-UCL
Response to wounding Source: BHF-UCL

Cellular Location

Extracellular space

Involvement in disease

Factor II deficiency (FA2D):
A very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels.
Ischemic stroke (ISCHSTR):
A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
Thrombophilia due to thrombin defect (THPH1):
The disease is caused by variants affecting the gene represented in this entry. A common genetic variation in the 3-prime untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increased risk of venous thrombosis. A multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation.
Pregnancy loss, recurrent, 2 (RPRGL2):
A common complication of pregnancy, resulting in spontaneous abortion before the fetus has reached viability. The term includes all miscarriages from the time of conception until 24 weeks of gestation. Recurrent pregnancy loss is defined as 3 or more consecutive spontaneous abortions.

PTM

The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).

Natasya, N., Tyniana, T. E. R. A., Savero, S., Swastika, N., & PARIKESIT, A. A. (2021). In silico computation of coagulation factor II: a potential water treatment agent against gram negative bacteria. Notulae Scientia Biologicae, 13(3), 11021-11021.

Tang, Y., Zhang, L., Xie, W., Jin, J., Luo, Y., Deng, M., ... & Fan, L. L. (2020). A Novel Heterozygous Variant in F2 Gene in a Chinese Patient With Coronary Thrombosis and Acute Myocardial Infarction Leads to Antithrombin Resistance. Frontiers in Genetics, 11, 184.

Takenouchi, T., Shimada, H., Uehara, T., Kanai, Y., Takahashi, T., & Kosaki, K. (2019). A paradoxical thrombogenic mutation in factor II at the target site of arthropod bleeding toxin. European Journal of Medical Genetics, 62(2), 93-95.

Zimowski, K. L., & Meeks, S. L. (2019). Factor II, Factor V, and Factor X Deficiencies. In Transfusion Medicine and Hemostasis (pp. 699-702). Elsevier.

Chinnaraj, M., Planer, W., & Pozzi, N. (2018). Structure of coagulation factor II: molecular mechanism of thrombin generation and development of next-generation anticoagulants. Frontiers in Medicine, 5, 281.

Farshi, Y., Dorgalaleh, A., & Tabibian, S. (2018). Congenital Factor II Deficiency. In Congenital Bleeding Disorders (pp. 183-199). Springer, Cham.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

F2 Matched Antibody Pair (396) (CAT#: APMAB-396LY)

Mouse Recombinant F2 protein, His Tag (CAT#: V2LY-0526-LY8142)

Antibody Pairs

F2 Matched Antibody Pair (396) (CAT#: APMAB-396LY)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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