Rat Recombinant JAM2 protein, hFc Tag (V2LY-0526-LY9497)

Name: Rat Recombinant JAM2 protein, hFc Tag
SKU: V2LY-0526-LY9497
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rat

Tag

hFc Tag

Protein Construction

This product is Rat Recombinant JAM2 protein, hFc Tag consist of Amino Acid: 1-236 and predicts a molecular mass of 50.3 kDa.

Molecule Mass

50.3 kDa

Sequence

Amino Acid: 1-236

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

junctional adhesion molecule 2

Function

Junctional adhesion protein that mediates heterotypic cell-cell interactions with its cognate receptor JAM3 to regulate different cellular processes (PubMed:11590146, PubMed:11823489, PubMed:24357068).
Plays a role in homing and mobilization of hematopoietic stem and progenitor cells within the bone marrow (PubMed:24357068).
At the surface of bone marrow stromal cells, it contributes to the retention of the hematopoietic stem and progenitor cells expressing JAM3 (PubMed:11590146, PubMed:24357068).
Plays a central role in leukocytes extravasation by facilitating not only transmigration but also tethering and rolling of leukocytes along the endothelium (PubMed:12239159).
Tethering and rolling of leukocytes are dependent on the binding by JAM2 of the integrin alpha-4/beta-1 (PubMed:12070135).
Plays a role in spermatogenesis where JAM2 and JAM3, which are respectively expressed by Sertoli and germ cells, mediate an interaction between both cell types and play an essential role in the anchorage of germ cells onto Sertoli cells and the assembly of cell polarity complexes during spermatid differentiation (By similarity).
Also functions as an inhibitory somatodendritic cue that prevents the myelination of non-axonal parts of neurons (By similarity).
During myogenesis, it is involved in myocyte fusion (By similarity).
May also play a role in angiogenesis (By similarity).

Biological Process

Cell-cell adhesionManual Assertion Based On ExperimentIMP:UniProtKB
Cellular extravasationManual Assertion Based On ExperimentIDA:UniProtKB
Hematopoietic stem cell migration to bone marrowManual Assertion Based On ExperimentIMP:UniProtKB
Leukocyte tethering or rollingManual Assertion Based On ExperimentIDA:ARUK-UCL
Lymphocyte aggregationManual Assertion Based On ExperimentIDA:ARUK-UCL
Maintenance of blood-brain barrier1 PublicationNAS:ARUK-UCL
Myoblast fusionISS:UniProtKB
Negative regulation of myelinationISS:UniProtKB
Positive regulation of lymphocyte migrationManual Assertion Based On ExperimentIDA:ARUK-UCL
Spermatid developmentISS:UniProtKB

Cellular Location

Cell membrane; Cell junction; Cell junction, tight junction. Localized at tight junctions of both epithelial and endothelial cells (By similarity).
Specifically localized within the somatodendritic compartment of neurons and excluded from the axon (By similarity).

Involvement in disease

Basal ganglia calcification, idiopathic, 8, autosomal recessive (IBGC8):
A form of basal ganglia calcification, a genetically heterogeneous condition characterized by symmetric calcification in the basal ganglia and other brain regions. Affected individuals can either be asymptomatic or show a wide spectrum of neuropsychiatric symptoms, including parkinsonism, dystonia, tremor, ataxia, dementia, psychosis, seizures, and chronic headache. Serum levels of calcium, phosphate, alkaline phosphatase and parathyroid hormone are normal. The neuropathological hallmark of the disease is vascular and pericapillary calcification, mainly of calcium phosphate, in the affected brain areas.

Topology

Extracellular: 29-238
Helical: 239-259
Cytoplasmic: 260-298

Wong, E. A., & Kinstler, S. R. (2023). Research Note: Junctional adhesion molecule A is expressed in epithelial cells of the crypt and villi whereas junctional adhesion molecule 2 is expressed in vascular cells. Poultry Science, 102(7), 102693.

Radulovic, V., van der Garde, M., Koide, S., Sigurdsson, V., Lang, S., Kaneko, S., & Miharada, K. (2019). Junctional adhesion molecule 2 represents a subset of hematopoietic stem cells with enhanced potential for T lymphopoiesis. Cell Reports, 27(10), 2826-2836.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rat Recombinant JAM2 protein, His Tag (CAT#: V2LY-0526-LY9498)

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Isotype control

For research use only. Not intended for any clinical use.

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