Rat Recombinant MDH1 protein, His Tag (V2LY-0526-LY9520)

Name: Rat Recombinant MDH1 protein, His Tag
SKU: V2LY-0526-LY9520
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Rat

Tag

His Tag

Protein Construction

This product is Rat Recombinant MDH1 protein, His Tag consist of Amino Acid: 4-334 and predicts a molecular mass of 38 kDa.

Molecule Mass

38 kDa

Sequence

Amino Acid: 4-334

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

MDH1

Function

Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH (PubMed:3052244).

Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation (PubMed:31538237).

Biological Process

Malate metabolic process Source: GO_Central
NADH metabolic process Source: GO_Central
Oxaloacetate metabolic process Source: GO_Central
Tricarboxylic acid cycle Source: GO_Central

Cellular Location

Cytoplasm

Involvement in disease

Developmental and epileptic encephalopathy 88 (DEE88):
A form of epileptic encephalopathy, a heterogeneous group of early-onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent. DEE88 is an autosomal recessive severe form characterized by global developmental delay, epilepsy, and progressive microcephaly.

PTM

ISGylated.
Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.

Kwon, H. J., Hahn, K. R., Kang, M. S., Choi, J. H., Moon, S. M., Yoon, Y. S., ... & Kim, D. W. (2023). Tat-malate dehydrogenase fusion protein protects neurons from oxidative and ischemic damage by reduction of reactive oxygen species and modulation of glutathione redox system. Scientific Reports, 13(1), 5653.

Thomas, M. J., Cassidy, E. R., Robinson, D. S., & Walstrom, K. M. (2022). Kinetic characterization and thermostability of C. elegans cytoplasmic and mitochondrial malate dehydrogenases. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1870(1), 140722.

Zhu, Q., Zhou, H., Wu, L., Lai, Z., Geng, D., Yang, W., ... & Yi, W. (2022). O-GlcNAcylation promotes pancreatic tumor growth by regulating malate dehydrogenase 1. Nature chemical biology, 18(10), 1087-1095.

Imran, M., Munir, M. Z., Ialhi, S., Abbas, F., Younus, M., Ahmad, S., ... & Shafiq, S. (2022). Identification and Characterization of Malate Dehydrogenases in Tomato (Solanum lycopersicum L.). International Journal of Molecular Sciences, 23(17), 10028.

McCue, W. M., & Finzel, B. C. (2021). Structural characterization of the human cytosolic malate dehydrogenase I. ACS omega, 7(1), 207-214.

Gu, H., Chen, C., Hao, X., Su, N., Huang, D., Zou, Y., ... & Zheng, J. (2020). MDH1-mediated malate-aspartate NADH shuttle maintains the activity levels of fetal liver hematopoietic stem cells. Blood, The Journal of the American Society of Hematology, 136(5), 553-571.

Nan, N., Wang, J., Shi, Y., Qian, Y., Jiang, L., Huang, S., ... & Xu, Z. Y. (2020). Rice plastidial NAD‐dependent malate dehydrogenase 1 negatively regulates salt stress response by reducing the vitamin B6 content. Plant Biotechnology Journal, 18(1), 172-184.

Jin, J., Huang, S., & Pan, J. (2019). A Novel Role for Malate Dehydrogenase 1 in Acute Myeloid Leukemia Progression. Blood, 134, 5048.

Broeks, M. H., Shamseldin, H. E., Alhashem, A., Hashem, M., Abdulwahab, F., Alshedi, T., ... & Alkuraya, F. S. (2019). MDH1 deficiency is a metabolic disorder of the malate–aspartate shuttle associated with early onset severe encephalopathy. Human genetics, 138, 1247-1257.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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