Rat Recombinant NPC2 protein, His Tag (V2LY-0526-LY9531)

Name: Rat Recombinant NPC2 protein, His Tag
SKU: V2LY-0526-LY9531
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rat

Tag

His Tag

Protein Construction

This product is Rat Recombinant NPC2 protein, His Tag consist of Amino Acid: 1-152 and predicts a molecular mass of 15.9 kDa.

Molecule Mass

15.9 kDa

Sequence

Amino Acid: 1-152

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

NPC Intracellular Cholesterol Transporter 2

Function

Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment (PubMed:17018531, PubMed:11125141, PubMed:18772377, PubMed:29580834, PubMed:15937921).
Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1 (PubMed:17018531, PubMed:18772377, PubMed:27238017).
May bind and mobilize cholesterol that is associated with membranes (PubMed:18823126).
NPC2 binds cholesterol with a 1:1 stoichiometry (PubMed:17018531).
Can bind a variety of sterols, including lathosterol, desmosterol and the plant sterols stigmasterol and beta-sitosterol (PubMed:17018531).
The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport (By similarity).

Biological Process

Cholesterol effluxManual Assertion Based On ExperimentIDA:BHF-UCL
Cholesterol homeostasisManual Assertion Based On ExperimentIDA:UniProtKB
Cholesterol metabolic processIEA:UniProtKB-KW
Cholesterol transportManual Assertion Based On ExperimentIDA:UniProtKB
Glycolipid transportManual Assertion Based On ExperimentTAS:HGNC-UCL
Intracellular cholesterol transportManual Assertion Based On ExperimentIDA:UniProtKB
Intracellular sterol transportManual Assertion Based On ExperimentIDA:HGNC-UCL
Phospholipid transportManual Assertion Based On ExperimentTAS:HGNC-UCL
Regulation of isoprenoid metabolic processManual Assertion Based On ExperimentTAS:UniProtKB
Response to virusManual Assertion Based On ExperimentIEP:UniProtKB
Sterol transportManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Secreted
Endoplasmic reticulum
Lysosome
Interaction with cell-surface M6PR mediates endocytosis and targeting to lysosomes.

Involvement in disease

Niemann-Pick disease C2 (NPC2):
A lysosomal storage disorder that affects the viscera and the central nervous system. It is due to defective intracellular processing and transport of low-density lipoprotein derived cholesterol. It causes accumulation of cholesterol in lysosomes, with delayed induction of cholesterol homeostatic reactions. Niemann-Pick disease type C2 has a highly variable clinical phenotype. Clinical features include variable hepatosplenomegaly and severe progressive neurological dysfunction such as ataxia, dystonia and dementia. The age of onset can vary from infancy to late adulthood.

Qian, H., Wu, X., Du, X., Yao, X., Zhao, X., Lee, J., ... & Yan, N. (2020). Structural basis of low-pH-dependent lysosomal cholesterol egress by NPC1 and NPC2. Cell, 182(1), 98-111.

Cologna, S. M., & Rosenhouse-Dantsker, A. (2019). Insights into the molecular mechanisms of cholesterol binding to the NPC1 and NPC2 proteins. Direct Mechanisms in Cholesterol Modulation of Protein Function, 139-160.

McCauliff, L. A., Langan, A., Li, R., Ilnytska, O., Bose, D., Waghalter, M., ... & Storch, J. (2019). Intracellular cholesterol trafficking is dependent upon NPC2 interaction with lysobisphosphatidic acid. Elife, 8, e50832.

Xu, Y., Zhang, Q., Tan, L., Xie, X., & Zhao, Y. (2019). The characteristics and biological significance of NPC2: Mutation and disease. Mutation Research/Reviews in Mutation Research, 782, 108284.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant NPC2 protein, His Tag (CAT#: V2LY-0526-LY5788)

Mouse Recombinant NPC2 protein, His Tag (CAT#: V2LY-0526-LY8708)

NPC2 Matched Antibody Pair (811) (CAT#: APMAB-811LY)

NPC2 Matched Antibody Pair (812) (CAT#: APMAB-812LY)

Antibody Pairs

NPC2 Matched Antibody Pair (811) (CAT#: APMAB-811LY)

NPC2 Matched Antibody Pair (812) (CAT#: APMAB-812LY)

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Rabbit Anti-NPC2 Recombinant Antibody (BA0189) (CAT#: CBMAB-0536CQ)

Mouse Anti-NPC2 Recombinant Antibody (1F4) (CAT#: CBMAB-N3112-WJ)

NPC2 Matched Antibody Pair (812) (CAT#: APMAB-812LY)

Mouse Recombinant NPC2 protein, His Tag (CAT#: V2LY-0526-LY8708)

NPC2 Matched Antibody Pair (811) (CAT#: APMAB-811LY)

Rabbit Anti-NPC2 Recombinant Antibody (CBWJN-1495) (CAT#: CBMAB-N3109-WJ)

Rabbit Anti-NPC2 Recombinant Antibody (BA0188) (CAT#: CBMAB-0535CQ)

Human Recombinant NPC2 protein, His Tag (CAT#: V2LY-0526-LY5788)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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