Rat Recombinant PDGFA protein, His Tag (V2LY-0526-LY9544)

Name: Rat Recombinant PDGFA protein, His Tag
SKU: V2LY-0526-LY9544
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rat

Tag

His Tag

Protein Construction

This product is Rat Recombinant PDGFA protein, His Tag consist of Amino Acid: 1-523 and predicts a molecular mass of 57.6 kDa.

Molecule Mass

57.6 kDa

Sequence

Amino Acid: 1-523

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Platelet Derived Growth Factor Subunit A

Function

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB (By similarity).

Biological Process

Actin cytoskeleton organizationISS:UniProtKB
AngiogenesisISS:UniProtKB
Animal organ morphogenesisISS:UniProtKB
Cell activationManual Assertion Based On ExperimentTAS:BHF-UCL
Cell projection assemblyISS:UniProtKB
Cell-cell signalingManual Assertion Based On ExperimentTAS:ProtInc
Cellular response to transforming growth factor beta stimulusIEA:Ensembl
Embryonic lung developmentISS:BHF-UCL
Hair follicle developmentISS:UniProtKB
Inner ear developmentIEA:Ensembl
Lung alveolus developmentISS:UniProtKB
Male gonad developmentIEA:Ensembl
Negative chemotaxisManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of phosphatidylinositol biosynthetic processManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of platelet activationManual Assertion Based On ExperimentIDA:BHF-UCL
Platelet-derived growth factor receptor signaling pathwayManual Assertion Based On ExperimentIDA:BHF-UCL
Platelet-derived growth factor receptor-alpha signaling pathway1 PublicationIC:ComplexPortal
Positive regulation of cell divisionIEA:UniProtKB-KW
Positive regulation of cell migrationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of cell population proliferationManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of ERK1 and ERK2 cascadeManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of fibroblast proliferationManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of MAP kinase activityManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of MAPK cascadeManual Assertion Based On ExperimentIMP:UniProtKB
Positive regulation of mesenchymal cell proliferationISS:UniProtKB
Positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of phosphatidylinositol 3-kinase signalingManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of protein autophosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of protein kinase B signalingManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of actin cytoskeleton organizationManual Assertion Based On ExperimentTAS:BHF-UCL
Regulation of branching involved in salivary gland morphogenesis by epithelial-mesenchymal signalingISS:UniProtKB
Regulation of peptidyl-tyrosine phosphorylationISS:UniProtKB
Regulation of smooth muscle cell migrationManual Assertion Based On ExperimentIDA:BHF-UCL
Response to estradiolIEA:Ensembl
Response to hypoxiaIEA:Ensembl
Response to inorganic substanceIEA:Ensembl
Response to retinoic acidIEA:Ensembl
Response to woundingManual Assertion Based On ExperimentIDA:BHF-UCL
Response to xenobiotic stimulusIEA:Ensembl
Skin developmentISS:UniProtKB
Wound healingManual Assertion Based On ExperimentTAS:BHF-UCL

Cellular Location

Secreted
Released by platelets upon wounding.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Canine Recombinant PDGFA protein, hFc Tag (CAT#: V2LY-0526-LY1304)

Canine Recombinant PDGFA protein, His Tag (CAT#: V2LY-0526-LY1305)

Human Recombinant PDGFA (D842V), Active protein, GST Tag (CAT#: V2LY-0526-LY6049)

Human Recombinant PDGFA (E675G, Y676C), Active protein, GST Tag (CAT#: V2LY-0526-LY6050)

Human Recombinant PDGFA protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY6051)

Human Recombinant PDGFA (G680R), Active protein, GST Tag (CAT#: V2LY-0526-LY6052)

Human Recombinant PDGFA, Active protein, GST Tag (CAT#: V2LY-0526-LY6053)

Human Recombinant PDGFA protein, hFc Tag (CAT#: V2LY-0526-LY6054)

Human Recombinant PDGFA protein, His Tag (CAT#: V2LY-0526-LY6055)

Human Recombinant PDGFA (T674I), Active protein, GST Tag (CAT#: V2LY-0526-LY6056)

Related Products

Human Recombinant PDGFA protein, hFc Tag (CAT#: V2LY-0526-LY6054)

Human Recombinant PDGFA (E675G, Y676C), Active protein, GST Tag (CAT#: V2LY-0526-LY6050)

Human Recombinant PDGFA protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY6051)

Mouse Anti-PDGFA Recombinant Antibody (CBYC-P255) (CAT#: CBMAB-P1243-YC)

Canine Recombinant PDGFA protein, hFc Tag (CAT#: V2LY-0526-LY1304)

Mouse Anti-PDGFA Recombinant Antibody (184805) (CAT#: CBMAB-P1241-YC)

Mouse Recombinant PDGFA protein, His Tag (CAT#: V2LY-0526-LY8751)

Human Recombinant PDGFA (T674I), Active protein, GST Tag (CAT#: V2LY-0526-LY6056)

Rat Recombinant PDGFA protein, His Tag-1 (CAT#: V2LY-0526-LY9547)

Mouse Anti-PDGFA Recombinant Antibody (CBT3421) (CAT#: V2LY-0625-LY1977)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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