Rhesus Recombinant EphB1 protein, His Tag (V2LY-0526-LY9711)

Name: Rhesus Recombinant EphB1 protein, His Tag
SKU: V2LY-0526-LY9711
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rhesus

Tag

His Tag

Protein Construction

This product is Rhesus Recombinant EphB1 protein, His Tag consist of Amino Acid: 1-540 and predicts a molecular mass of 59.9 kDa.

Molecule Mass

59.9 kDa

Sequence

Amino Acid: 1-540

Species

Rhesus

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

EPH Receptor B1

Research Area

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively. Involved in the maintenance of the pool of satellite cells (muscle stem cells) by promoting their self-renewal and reducing their activation and differentiation (By similarity).

Biological Process

Angiogenesis Source: UniProtKB
Axon guidance Source: UniProtKB
Camera-type eye morphogenesis Source: Ensembl
Cell chemotaxis Source: UniProtKB
Cell-substrate adhesion Source: UniProtKB
Central nervous system projection neuron axonogenesis Source: UniProtKB
Dendritic spine development Source: UniProtKB
Dendritic spine morphogenesis Source: UniProtKB
Detection of temperature stimulus involved in sensory perception of pain Source: UniProtKB
Ephrin receptor signaling pathway Source: UniProtKB
Establishment of cell polarity Source: UniProtKB
Immunological synapse formation Source: Ensembl
Modulation of chemical synaptic transmission Source: Ensembl
Negative regulation of satellite cell differentiation Source: UniProtKB
Negative regulation of skeletal muscle satellite cell proliferation Source: UniProtKB
Neural precursor cell proliferation Source: UniProtKB
Neurogenesis Source: UniProtKB
Optic nerve morphogenesis Source: Ensembl
Positive regulation of kinase activity Source: GO_Central
Positive regulation of synapse assembly Source: UniProtKB
Protein autophosphorylation Source: UniProtKB
Regulation of ERK1 and ERK2 cascade Source: UniProtKB
Regulation of JNK cascade Source: UniProtKB
Regulation of neuron death Source: UniProtKB
Retinal ganglion cell axon guidance Source: UniProtKB
Skeletal muscle satellite cell activation Source: UniProtKB
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central

Cellular Location

Early endosome membrane; Cell membrane; Dendrite

Topology

Extracellular: 18-540
Helical: 541-563
Cytoplasmic: 564-984

PTM

Phosphorylated. Autophosphorylation is stimulated by the ligand EFNB1. Required for interaction with SH2 domain-containing interactors, for activation of the MAPK/ERK and JUN signaling cascades and for ubiquitination by CBL.
Ubiquitinated; (EFNB1)ligand-induced poly- and/or multi-ubiquitination by CBL is regulated by SRC and leads to lysosomal degradation.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant EphB1 protein, GST Tag (CAT#: V2LY-0526-LY3895)

Human Recombinant EphB1 protein, His Tag (CAT#: V2LY-0526-LY3896)

Mouse Recombinant EphB1, Active protein, GST Tag (CAT#: V2LY-0526-LY8249)

Mouse Recombinant EphB1 protein, GST Tag (CAT#: V2LY-0526-LY8250)

Mouse Recombinant EphB1 protein, His Tag (CAT#: V2LY-0526-LY8251)

Rhesus Recombinant EphB1 protein, hFc Tag (CAT#: V2LY-0526-LY9710)

Related Products

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Mouse Anti-EPHB1 Recombinant Antibody (CBT488) (CAT#: V2LY-0625-LY3484)

Mouse Anti-EPHB1 Recombinant Antibody (CBFYE-0999) (CAT#: CBMAB-E1497-FY)

Rhesus Recombinant EphB1 protein, hFc Tag (CAT#: V2LY-0526-LY9710)

Mouse Anti-EPHB1 Recombinant Antibody (CBFYE-1001) (CAT#: CBMAB-E1499-FY)

Mouse Anti-EPHB1 Recombinant Antibody (4G6) (CAT#: CBMAB-A2652-LY)

Human Recombinant EphB1 protein, His Tag (CAT#: V2LY-0526-LY3896)

Rabbit Anti-EPHB1 Recombinant Antibody (CBFYC-2612) (CAT#: CBMAB-C2686-FY)

Mouse Recombinant EphB1, Active protein, GST Tag (CAT#: V2LY-0526-LY8249)

Mouse Anti-EPHB1 Recombinant Antibody (CBFYE-1002) (CAT#: CBMAB-E1502-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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