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Mouse Anti-Skp2 Recombinant Antibody (4D8-B9-F3) (CBMAB-Z0271-LY)

The product is antibody recognizes Skp2. The antibody 4D8-B9-F3 immunoassay techniques such as: WB.
See all Skp2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4D8-B9-F3
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
Purified recombinant fragment of SKP2 (aa1-130) expressed in E. Coli.
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
S-Phase Kinase Associated Protein 2
Entrez Gene ID
6502
UniProt ID
Q13309
Alternative Names
FBL1; FLB1; FBXL1; MGC1366; SKP2
Function
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription (PubMed:11931757, PubMed:12435635, PubMed:12769844, PubMed:12840033, PubMed:15342634, PubMed:15668399, PubMed:15949444, PubMed:16103164, PubMed:16262255, PubMed:16581786, PubMed:16951159, PubMed:17908926, PubMed:17962192, PubMed:22770219, PubMed:32267835).
Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition (By similarity).
Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, YTHDF2, and probably MYC, TOB1 and TAL1 (PubMed:11931757, PubMed:12435635, PubMed:12769844, PubMed:12840033, PubMed:15342634, PubMed:15668399, PubMed:15949444, PubMed:16103164, PubMed:17962192, PubMed:16581786, PubMed:16951159, PubMed:17908926, PubMed:32267835).
Degradation of TAL1 also requires STUB1 (PubMed:17962192).
Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2 (PubMed:16262255).
Promotes ubiquitination and destruction of CDH1 in a CK1-dependent manner, thereby regulating cell migration (PubMed:22770219).
Through the ubiquitin-mediated proteasomal degradation of hepatitis C virus non-structural protein 5A, has an antiviral activity towards that virus.
Biological Process
Biological Process defense response to virusManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process G1/S transition of mitotic cell cycleManual Assertion Based On ExperimentTAS:ProtInc
Biological Process G2/M transition of mitotic cell cycleManual Assertion Based On ExperimentIBA:GO_Central
Biological Process innate immune responseManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of intracellular estrogen receptor signaling pathwayIEA:Ensembl
Biological Process positive regulation of protein polyubiquitinationIEA:Ensembl
Biological Process proteasome-mediated ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein K48-linked ubiquitinationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of apoptotic processManual Assertion Based On ExperimentIDA:MGI
Biological Process regulation of cell cycleManual Assertion Based On ExperimentIBA:GO_Central
Biological Process SCF-dependent proteasomal ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cytoplasm
Nucleus
PTM
Ubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Deubiquitinated by USP13.
Acetylation at Lys-68 and Lys-71 increases stability through impairment of APC/C-mediated proteolysis and promotes cytoplasmic retention. Deacetylated by SIRT3.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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