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Human Stem Cell Biomarker Antibody Array (Glass Slide, 15 Targets) (Y0572) (AbAr-0572-YC)

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Specifications

Target Array
Stem Cell Array
Target
Alpha-fetoprotein, OCT-4, BMPR-IA, ALK-6, Brachyury, CD38, E-Cadherin, GATA4, hCG beta, Nanog, Nestin, PDX-1, SOX2, SOX17, VEGFR2
Species Reactivity
Human
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Glass Slide
Detection Assay
Sandwich-based Assay
Protocol Outline
Conduct surface blocking of the glass slide followed by sample incubation.
Incubate with a biotinylated antibody cocktail, followed by incubation with fluorescent dye-conjugated streptavidin.
Utilize a scanner to analyze the antibody array.
Components
Antibody Array Slide
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction
Stem cells are cells that can differentiate into other types of cells, and can also divide in self-renewal to produce more of the same type of stem cells. In mammals, there are two broad types of stem cells: embryonic stem cells, which are isolated from the inner cell mass of blastocysts in early embryonic development, and adult stem cells, which are found in various tissues of fully developed mammals. In adult organisms, stem cells and progenitor cells act as a repair system for the body, replenishing adult tissues. In a developing embryo, stem cells can differentiate into all the specialized cells—ectoderm, endoderm and mesoderm (see induced pluripotent stem cells)—but also maintain the normal turnover of regenerative organs, such as blood, skin, or intestinal tissues.
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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