GST
Genetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the FLAG-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.
Full Name
GST
Anti-GST antibodies
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CompareTarget: GST
Host: Mouse
Antibody Isotype: IgG
Specificity: Human
Clone: CBLG1-1938
Application*: WB
Target: GST
Host: Mouse
Antibody Isotype: IgG
Specificity: All
Clone: 6H6
Application*: E, WB
Target: GST
Host: Mouse
Antibody Isotype: IgG
Specificity: All
Clone: 1B5
Application*: E, WB
Target: GST
Host: Mouse
Antibody Isotype: IgG1
Specificity: All
Clone: CAP864
Application*: ELISA, WB
Target: GST
Host: Mouse
Antibody Isotype: IgG1
Specificity: All
Clone: CAP863
Application*: ELISA, WB
Target: GST
Host: Mouse
Antibody Isotype: IgG1
Specificity: All
Clone: CAP862
Application*: ELISA, IP, WB
Target: GST
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 6G9C6, 6G9B9
Application*: WB
Target: GST
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 5C12
Application*: CI, E, IH, WB
Target: GST
Host: Mouse
Antibody Isotype: IgG1
Specificity: S. japonicum
Clone: 6D420
Application*: E, WB
Target: GST
Host: Mouse
Antibody Isotype: IgG1
Specificity: S. japonicum
Clone: 6C10G4
Application*: WB
Target: GST
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: S. japonicum
Clone: 6A91GST3-4C
Application*: E, IP, WB
Target: GST
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: S. japonicum
Clone: 6A91
Application*: E, WB
Target: GST
Host: Mouse
Antibody Isotype: IgG
Specificity: S. japonicum
Clone: 4H327
Application*: WB
Target: GST
Host: Mouse
Antibody Isotype: IgG2a
Specificity: S. japonicum
Clone: 2G10B6
Application*: E
Target: GST
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: S. japonicum
Clone: 14B18
Application*: E, IH, WB
Target: GST
Host: Mouse
Antibody Isotype: IgG2a
Specificity: S. japonicum
Clone: 13G6
Application*: E, IC, IP, WB
Target: GST
Host: Rat
Antibody Isotype: IgG2a
Specificity: Rat
Clone: 10K217KT4
Application*: E, WB
Target: GST
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Rat
Clone: 10K217
Application*: E, WB
Target: GST
Host: Mouse
Antibody Isotype: IgG
Specificity: S. japonicum
Clone: 5C116C7
Application*: E
Target: GST
Host: Mouse
Antibody Isotype: IgG
Specificity: S. japonicum
Clone: 11C106
Application*: E, WB
Target: GST
Host: Mouse
Antibody Isotype: IgG1
Specificity: Chemical
Clone: vpg66
Application*: IF, WB
Target: GST
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Chemical
Clone: CBFYH-2987
Application*: E, WB
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For Research Use Only. Not For Clinical Use.
(P): Predicted
* Abbreviations
- AActivation
- AGAgonist
- APApoptosis
- BBlocking
- BABioassay
- BIBioimaging
- CImmunohistochemistry-Frozen Sections
- CIChromatin Immunoprecipitation
- CTCytotoxicity
- CSCostimulation
- DDepletion
- DBDot Blot
- EELISA
- ECELISA(Cap)
- EDELISA(Det)
- ESELISpot
- EMElectron Microscopy
- FFlow Cytometry
- FNFunction Assay
- GSGel Supershift
- IInhibition
- IAEnzyme Immunoassay
- ICImmunocytochemistry
- IDImmunodiffusion
- IEImmunoelectrophoresis
- IFImmunofluorescence
- IGImmunochromatography
- IHImmunohistochemistry
- IMImmunomicroscopy
- IOImmunoassay
- IPImmunoprecipitation
- ISIntracellular Staining for Flow Cytometry
- LALuminex Assay
- LFLateral Flow Immunoassay
- MMicroarray
- MCMass Cytometry/CyTOF
- MDMeDIP
- MSElectrophoretic Mobility Shift Assay
- NNeutralization
- PImmunohistologyp-Paraffin Sections
- PAPeptide Array
- PEPeptide ELISA
- PLProximity Ligation Assay
- RRadioimmunoassay
- SStimulation
- SESandwich ELISA
- SHIn situ hybridization
- TCTissue Culture
- WBWestern Blot
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